Hansen-Smith F M, Watson L, Lu D Y, Goldstein I
Department of Biological Sciences, Oakland University, Rochester, Michigan 48309-4401.
Microvasc Res. 1988 Nov;36(3):199-215. doi: 10.1016/0026-2862(88)90022-2.
Previous studies on mice have revealed that the Griffonia simplicifolia I (GSI) lectin selectively binds to capillaries in a number of microvascular beds. These observations suggest that the lectin might be a suitable microvascular marker for physiological studies of skeletal muscle, particularly when fluorescent visualization of vessels is desired independently of their perfusion status. Since species and strain heterogeneity has been demonstrated for certain lectins associated with the microcirculatory vessels, lectin binding was studied in a number of muscles taken from the major species of mammals used for experimental purposes. Staining of cryostat sections confirmed the utility of GSI as a marker for capillaries from muscle of mice, rats, hamsters, rabbits, dogs, and monkeys. Differential staining of arterioles and veins was revealed by double labeling with GSI and antisera to Factor VIII-related antigen. Double labeling for GSI binding and alkaline phosphatase activity revealed that the GSI method detects many more capillaries and terminal arterioles than does the alkaline phosphatase method. GSI binding to unfixed whole mounts of thin skeletal muscles (hamster cheek pouch, mouse diaphragm, and rat cremaster) was studied to determine whether the GSI lectin would be a suitable marker for intravital studies. An extensive microvascular bed, including terminal arterioles, venules, and capillaries, was revealed which could be visualized in the complete absence of perfusion with fluorescent markers. These observations suggest that the GSI lectin may be extremely useful as a probe for the microcirculation of skeletal muscle in many types of physiological experiments.
先前对小鼠的研究表明，西非吊灯树凝集素I（GSI）可选择性地与多个微血管床中的毛细血管结合。这些观察结果表明，该凝集素可能是骨骼肌生理学研究中合适的微血管标记物，特别是当需要独立于血管灌注状态对血管进行荧光可视化时。由于已证明与微循环血管相关的某些凝集素存在物种和品系异质性，因此对用于实验目的的主要哺乳动物物种的多种肌肉中的凝集素结合情况进行了研究。低温恒温器切片染色证实了GSI作为小鼠、大鼠、仓鼠、兔子、狗和猴子肌肉中毛细血管标记物的实用性。通过用GSI和抗VIII因子相关抗原的抗血清进行双重标记，揭示了小动脉和静脉的差异染色。GSI结合与碱性磷酸酶活性的双重标记显示，与碱性磷酸酶方法相比，GSI方法检测到的毛细血管和终末小动脉更多。研究了GSI与薄骨骼肌（仓鼠颊囊、小鼠膈肌和大鼠提睾肌）未固定的整装标本的结合情况，以确定GSI凝集素是否适合用于活体研究。结果显示出一个广泛的微血管床，包括终末小动脉、小静脉和毛细血管，在完全没有荧光标记物灌注的情况下也可以可视化。这些观察结果表明，在许多类型的生理学实验中，GSI凝集素作为骨骼肌微循环的探针可能极其有用。
