Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish.
作者信息
Ikeda Takeshi, Ichikawa Kohei, Shigeto Hajime, Ishida Takenori, Hirota Ryuichi, Funabashi Hisakage, Kuroda Akio
机构信息
Unit of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
出版信息
Biosci Biotechnol Biochem. 2019 Dec;83(12):2272-2275. doi: 10.1080/09168451.2019.1659716. Epub 2019 Sep 4.
Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.
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