Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Institute of Physical Chemistry, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Analyst. 2019 Oct 7;144(19):5842-5847. doi: 10.1039/c9an01412h. Epub 2019 Sep 4.
Depurination occurs via hydrolysis of the purine-deoxyribose glycosyl bond and causes nucleic acid damage. In particular, the DNA sequences that can undergo a self-catalyzed depurination (SCD) will cause a great uncertainty in duplicating, separating, purifying, and storing the DNA samples. Therefore, there is a great demand to develop a rapid detection method for SCD events. Herein, the use of a convenient fluorescence method to follow the site-specific SCD was demonstrated. We found that the resultant apurine site (AP site) from depurination can be selectively recognized by a fluorescent probe of palmatine (PAL) with a turn-on fluorescence response. The dependence of SCD on the bases of the depurination site, pH, metal ions, and time shows that our method can be used to rapidly evaluate the depurination process. Furthermore, the depurination process can be photo-switched using a photoacid as an external initiator. Our work will find wide applications in preliminarily identifying the DNA depurination.
脱嘌呤是通过嘌呤-脱氧核糖糖苷键的水解发生的，会导致核酸损伤。特别是，能够进行自我催化脱嘌呤（SCD）的 DNA 序列会给 DNA 样本的复制、分离、纯化和储存带来极大的不确定性。因此，人们对开发 SCD 事件的快速检测方法有很大的需求。在此，我们展示了一种利用方便的荧光法来跟踪特定位置 SCD 的方法。我们发现，脱嘌呤产生的无嘌呤部位（AP 部位）可以被小檗碱（PAL）的荧光探针选择性识别，产生开启型荧光响应。SCD 对脱嘌呤部位碱基、pH 值、金属离子和时间的依赖性表明，我们的方法可用于快速评估脱嘌呤过程。此外，脱嘌呤过程可以用光酸作为外部引发剂进行光切换。我们的工作将在初步鉴定 DNA 脱嘌呤方面有广泛的应用。
