Mogami Veterinary Clinical Center, Yamagata Prefectural Federated Agricultural Mutual Aid Association, 260 Ipponyanagi, Matsumotoaza, Shinjo, Yamagata, 996-0051, Japan.
Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
Vet Res Commun. 2019 Nov;43(4):215-224. doi: 10.1007/s11259-019-09760-0. Epub 2019 Sep 5.
The objective of this study was to evaluate blood levels of various hormones and compounds related to energy metabolism in cows with subacute ruminal acidosis (SARA). We investigated 11 lactating cows presumed to have SARA based on duration of ruminal pH <5.6 and reticulum pH <6.3 in 2015-2016. Kraft pulp (KP) was used to supplement feed of 7 of the cows studied in an effort to reduce SARA. We continuously monitored ruminal pH and measured blood concentrations of hormones and metabolites related to energy metabolism. Blood measurements included glucose (GLU), total cholesterol (TC), free fatty acid (FFA), insulin, adiponectin (ADN), malate dehydrogenase (MDH), and lactate dehydrogenase (LDH). Additionally, we analyzed milk data (milk yield, milk fat percentage, milk protein percentage, milk urea nitrogen, and protein fat ratio) and reproduction data. The results demonstrated that ADN levels at 4 weeks post-parturition correlated with the total amount of time that the ruminal or reticulum fluid pH was under the threshold during 1 week post-parturition, as well as the numbers of days the cows were diagnosed with SARA (SARA-positive days) up to 30 days post-parturition. SARA-positive days in 2016 were higher than those in 2015. In both years, numbers of SARA-positive days for cows supplemented with KP were lower than those for cows without KP. Increased ADN levels may be a compensatory reaction to frequent SARA which modulates the inflammatory response against high LPS levels and improves insulin resistance caused by LPS. ADN may serve as an estimative index for SARA.
本研究旨在评估患有亚急性瘤胃酸中毒（SARA）奶牛的血液中各种与能量代谢相关的激素和化合物水平。我们对 2015-2016 年间被认为患有 SARA 的 11 头泌乳奶牛进行了研究，这些奶牛的瘤胃 pH 值<5.6 和网胃 pH 值<6.3 的持续时间较长。我们使用硫酸盐浆（KP）为 7 头研究奶牛补充饲料，以减少 SARA。我们连续监测瘤胃 pH 值，并测量与能量代谢相关的激素和代谢物的血液浓度。血液测量包括葡萄糖（GLU）、总胆固醇（TC）、游离脂肪酸（FFA）、胰岛素、脂联素（ADN）、苹果酸脱氢酶（MDH）和乳酸脱氢酶（LDH）。此外，我们还分析了牛奶数据（产奶量、乳脂百分比、乳蛋白百分比、牛奶尿素氮和蛋白质脂肪比）和繁殖数据。结果表明，产后 4 周的 ADN 水平与产后 1 周内瘤胃或网胃液体 pH 值低于阈值的总时间以及奶牛被诊断为 SARA 的天数（SARA 阳性天数）相关，直至产后 30 天。2016 年的 SARA 阳性天数高于 2015 年。在这两年中，补充 KP 的奶牛的 SARA 阳性天数少于没有补充 KP 的奶牛。ADN 水平的升高可能是对频繁发生的 SARA 的一种代偿反应，这种反应可以调节对高 LPS 水平的炎症反应，并改善 LPS 引起的胰岛素抵抗。ADN 可以作为 SARA 的估计指标。
