The Gastrointestinal Surgery of the Second Affiliated Hospital of Guilin Medical College, GuiLin 541199, Guilin City, Guangxi Zhuang Autonomous Region, PR China.
J Nanosci Nanotechnol. 2020 Mar 1;20(3):1366-1374. doi: 10.1166/jnn.2020.17362.
Epithelial-mesenchymal transition (EMT) is an important factor in promoting the metastasis of colon cancer, which leads to clinical incurability. It has been found that bone morphogenetic proteins (BMPs) are closely related to EMT and the prognoses of most malignant tumors, including colon cancer tumors. However, the effects and mechanisms of BMP1 on the EMT of colon cancer are not yet clear. To explore the effects and mechanisms of BMP1 on the EMT of colon cancer, a BMP1 overexpression plasmid vector was used to interfere with SW620 cells and real-time fluorescence quantitative RNA and western blotting were used to detect the effects of BMP1 on the transcription and translation of COL1A1 and COL1A2 genes, as well as EMT-related genes, including beta-catenin, vimentin, and E-cadherin (E-Cad) genes in SW620 cells. MTT assay and Transwell techniques were used to detect the effects of BMP1 on the proliferation and migration of SW620 cells. The results demonstrate that BMP1 expression in SW620 cells is significantly lower than that in HCoEpiC cells, which promotes the expression of COL1A1 and COL1A2. Additionally, the expression of genes related to EMT, including beta-catenin and vimentin, increased, whereas E-Cad expression decreased. This difference was significant, which led to an increase in cell viability and the number of migrating cells in SW620 cells. Based on the overexpression of BMP1, the expression of COL1A1 and COL1A2 in SW620 was inhibited, which inhibited the process of EMT. Specifically, vimentin expression decreased, and E-Cad and beta-catenin expression increased. Additionally, SW620 cell viability decreased and migration ability decreased. Therefore, it can be concluded that the absence of BMP1 promotes the expression of COL1A and COL1A2 in colon cancer and promotes the process of EMT. Increasing the expression of BMP1 can inhibit the process of EMT in colon cancer, thereby inhibiting the migration of tumors.
上皮-间充质转化（EMT）是促进结肠癌转移的重要因素，导致临床不可治愈。已经发现骨形态发生蛋白（BMPs）与 EMT 以及大多数恶性肿瘤（包括结肠癌）的预后密切相关。然而，BMP1 对结肠癌 EMT 的影响和机制尚不清楚。为了探讨 BMP1 对结肠癌 EMT 的影响和机制，使用 BMP1 过表达质粒载体干扰 SW620 细胞，实时荧光定量 RNA 和 Western blot 检测 BMP1 对 SW620 细胞 COL1A1 和 COL1A2 基因转录和翻译以及 EMT 相关基因，包括β-连环蛋白、波形蛋白和 E-钙粘蛋白（E-Cad）基因的影响。MTT 检测和 Transwell 技术用于检测 BMP1 对 SW620 细胞增殖和迁移的影响。结果表明，SW620 细胞中的 BMP1 表达明显低于 HCoEpiC 细胞，促进了 COL1A1 和 COL1A2 的表达。此外，EMT 相关基因，包括β-连环蛋白和波形蛋白的表达增加，而 E-Cad 表达减少。这种差异具有统计学意义，导致 SW620 细胞活力增加和迁移细胞数量增加。基于 BMP1 的过表达，SW620 中 COL1A1 和 COL1A2 的表达受到抑制，从而抑制了 EMT 过程。具体来说，波形蛋白表达减少，E-Cad 和β-连环蛋白表达增加。此外，SW620 细胞活力降低，迁移能力降低。因此，可以得出结论，BMP1 的缺失促进了结肠癌中 COL1A 和 COL1A2 的表达，并促进了 EMT 过程。增加 BMP1 的表达可以抑制结肠癌中 EMT 的发生，从而抑制肿瘤的迁移。
