Beijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Reproduction. 2019 Nov;158(5):415-427. doi: 10.1530/REP-19-0081.
NLRP (NACHT, LRR and PYD domain-containing proteins) family plays pivotal roles in mammalian reproduction. Mutation of NLRP7 is often associated with human recurrent hydatidiform moles. Few studies regarding the functions of NLRP7 have been performed in other mammalian species rather than humans. In the current study, for the first time, the function of NLRP7 has been explored in ovine ovary. NLRP7 protein was mainly located in ovarian follicles and in in vitro pre-implantation embryos. To identify its origin, 763 bp partial CDS of NLRP7 deriving from sheep cumulus oocyte complexes (COCs) was cloned, it showed a great homology with Homo sapiens. The high levels of mRNA and protein of NLRP7 were steadily expressed in oocytes, parthenogenetic embryos or IVF embryos. NLRP7 knockdown by the combination of siRNA and shRNA jeopardized both the parthenogenetic and IVF embryo development. These results strongly suggest that NLRP7 plays an important role in ovine reproduction. The potential mechanisms of NLRP7 will be fully investigated in the future.
NLRP（NACHT、LRR 和 PYD 结构域蛋白）家族在哺乳动物生殖中发挥着关键作用。NLRP7 的突变常与人类复发性葡萄胎有关。目前，除人类外，对其他哺乳动物 NLRP7 的功能研究较少。在本研究中，首次探索了 NLRP7 在绵羊卵巢中的功能。NLRP7 蛋白主要定位于卵巢卵泡和体外着床前胚胎中。为了确定其来源，我们克隆了来自绵羊卵丘卵母细胞复合体（COCs）的 NLRP7 的 763bp 部分 CDS，它与 Homo sapiens 具有高度同源性。NLRP7 的 mRNA 和蛋白水平在卵母细胞、孤雌激活胚胎或体外受精胚胎中均稳定表达。siRNA 和 shRNA 的组合敲低 NLRP7 会危及孤雌激活胚胎和体外受精胚胎的发育。这些结果强烈表明 NLRP7 在绵羊生殖中发挥重要作用。未来将全面研究 NLRP7 的潜在机制。
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