Allicin Inhibited -Induced Mastitis by Reducing Lipid Raft Stability via LxRα in Mice.

Mastitis, inflammation of the mammary gland, occurs in both humans and animals. is the most common infectious bacterial pathogen associated with mastitis. We investigated the effects of allicin on -induced mastitis in mice. Pathological histology revealed that allicin inhibited -induced pathological damage and myeloperoxidase activity in mammary tissues. Enzyme-linked immunosorbent assays demonstrated that allicin reduced the production of IL-1β and TNF-α as well as inhibited the NF-κB and mitogen-activated protein kinase pathway by reducing phosphorylation of p65, IκBα, p38, JNK, and ERK. Western blotting revealed that allicin reduced TLR2 and TLR6 expression in mammary tissues and cells but not in HEK293 cells. The lipid raft content was reduced by allicin, which inhibited signaling downstream of TLR2 and TLR6. Liver X receptor α (α) luciferase reporter assays and α interference experiments showed that allicin improved the α activity and adenosine 5'-triphosphate-binding cassette G and A1 (ABCG and ABCA1) expression, thereby reducing the cholesterol level, lipid raft formation, and downstream TLR2 and TLR6 pathway activity. These results demonstrated that allicin exerted anti-inflammatory effects against mastitis by improving the α activity and reducing lipid raft formation.