Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou, China.
Nat Commun. 2019 Sep 13;10(1):4164. doi: 10.1038/s41467-019-12118-4.
BES1 and BZR1 were originally identified as two key transcription factors specifically regulating brassinosteroid (BR)-mediated gene expression. They belong to a family consisting of six members, BES1, BZR1, BEH1, BEH2, BEH3, and BEH4. bes1 and bzr1 single mutants do not exhibit any characteristic BR phenotypes, suggesting functional redundancy of these proteins. Here, by generating higher order mutants, we show that a quintuple mutant is male sterile due to defects in tapetum and microsporocyte development in anthers. Our genetic and biochemical analyses demonstrate that BES1 family members also act as downstream transcription factors in the EMS1-TPD1-SERK1/2 pathway. Ectopic expression of both TPD1 and EMS1 in bri1-116, a BR receptor null mutant, leads to the accumulation of non-phosphorylated, active BES1, similar to activation of BES1 by BRI1-BR-BAK1 signaling. These data suggest that two distinctive receptor-like kinase-mediated signaling pathways share BES1 family members as downstream transcription factors to regulate different aspects of plant development.
BES1 和 BZR1 最初被鉴定为两个关键的转录因子，专门调节油菜素内酯 (BR) 介导的基因表达。它们属于一个由六个成员组成的家族，包括 BES1、BZR1、BEH1、BEH2、BEH3 和 BEH4。bes1 和 bzr1 单突变体不表现出任何特征性的 BR 表型，表明这些蛋白质具有功能冗余性。在这里，通过生成更高阶的突变体，我们发现五重突变体由于花药中绒毡层和小孢子母细胞发育缺陷而导致雄性不育。我们的遗传和生化分析表明，BES1 家族成员也作为 EMS1-TPD1-SERK1/2 途径中的下游转录因子发挥作用。在 BR 受体缺失突变体 bri1-116 中异位表达 TPD1 和 EMS1 会导致非磷酸化、活性 BES1 的积累，类似于 BRI1-BR-BAK1 信号转导对 BES1 的激活。这些数据表明，两种独特的受体样激酶介导的信号通路共享 BES1 家族成员作为下游转录因子，以调节植物发育的不同方面。
Zotero 插件
