Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Minas Gerais, CEP 33600-000, Brazil.
Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Arch Virol. 2019 Dec;164(12):3045-3050. doi: 10.1007/s00705-019-04382-y. Epub 2019 Sep 13.
Infection with ovine gammaherpesvirus 2 (OvHV-2) is generally asymptomatic in sheep; however, when it crosses the species barrier, it causes malignant catarrhal fever (MCF) in cattle. In the present study, we developed a real-time PCR assay and a droplet digital PCR assay and use both methods to study an outbreak caused by OvHV-2. Both PCR methods showed high sensitivity and specificity and were able to detect low copy numbers of OvHV-2 in sheep and cattle. The present study describes the first digital PCR quantification of OvHV-2 genome copies in samples collected from sheep and cattle.
绵羊感染绵羊γ-疱疹病毒 2（OvHV-2）通常无症状；然而，当它跨越物种屏障时，会导致牛恶性卡他热（MCF）。在本研究中，我们开发了一种实时 PCR 检测方法和一种数字 PCR 检测方法，并使用这两种方法研究了由 OvHV-2 引起的一次暴发。两种 PCR 方法均表现出较高的灵敏度和特异性，并且能够检测绵羊和牛中低拷贝数的 OvHV-2。本研究描述了首次对来自绵羊和牛的样本中 OvHV-2 基因组拷贝进行数字 PCR 定量。
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