急性心肌梗死患者血清蛋白中丙二酰化、琥珀酰化和戊二酰化的鉴定
Identification of Malonylation, Succinylation, and Glutarylation in Serum Proteins of Acute Myocardial Infarction Patients.
作者信息
Zhou Boda, Du Yipeng, Xue Yajun, Miao Guobin, Wei Taotao, Zhang Ping
机构信息
Department of Cardiology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University, Beijing, 102218, China.
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
出版信息
Proteomics Clin Appl. 2020 Jan;14(1):e1900103. doi: 10.1002/prca.201900103. Epub 2019 Nov 18.
PURPOSE
To identify protein malonylation, succinylation, and glutarylation in human and rat serum.
EXPERIMENTAL DESIGN
Immunoprecipitation coupled with MS/MS is employed to compare the relative abundance of malonylation, succinylation, and glutarylation of serum protein in acute myocardial infarction human and rat.
RESULTS
One hundred thirty and 48 unique malonylated, succinylated, or glutarylated peptides are found in human and rat serum, respectively. Succinylation is the most predominant modification. The most modified protein is albumin. Abundance of serum protein succinylation and glutarylation is significantly (p < 0.05) lower in the peripheral serum of ST-segment elevation myocardial infarction patients compared with healthy volunteers, which is also observed in acute myocardial infarction rats.
CONCLUSIONS AND CLINICAL RELEVANCE
Malonylation, succinylation, and glutarylation widely exist in mammalian serum proteins, and may reveal novel mechanism of acute myocardial infarction.
目的
鉴定人和大鼠血清中的蛋白质丙二酰化、琥珀酰化和戊二酰化修饰。
实验设计
采用免疫沉淀结合串联质谱法，比较急性心肌梗死患者和大鼠血清中蛋白质丙二酰化、琥珀酰化和戊二酰化修饰的相对丰度。
结果
在人血清和大鼠血清中分别发现了130个和48个独特的丙二酰化、琥珀酰化或戊二酰化修饰的肽段。琥珀酰化修饰最为常见。修饰最多的蛋白质是白蛋白。与健康志愿者相比，ST段抬高型心肌梗死患者外周血清中蛋白质琥珀酰化和戊二酰化修饰的丰度显著降低（p < 0.05），急性心肌梗死大鼠中也观察到了这一现象。
结论及临床意义
丙二酰化、琥珀酰化和戊二酰化修饰广泛存在于哺乳动物血清蛋白中，可能揭示急性心肌梗死的新机制。
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