1 型发作性睡病中神经退行性变的脑脊液生物标志物。

Cerebrospinal fluid biomarkers of neurodegeneration in narcolepsy type 1.

机构信息

Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.

IRCCS, Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy.

出版信息

Sleep. 2020 Feb 13;43(2). doi: 10.1093/sleep/zsz215.

DOI:10.1093/sleep/zsz215

PMID:31552425

Abstract

STUDY OBJECTIVES

To measure the levels of five neurodegenerative biomarkers in the cerebrospinal fluid (CSF) of patients with narcolepsy type 1 (NT1) with variable disease duration.

METHODS

Following a standardized protocol of CSF collection and storage, we measured CSF total- and phosphorylated-tau, amyloid-beta 1-40 and 1-42, and neurofilament light chain (NfL) proteins in 30 nonneurological controls and 36 subjects with NT1, including 14 patients with recent disease onset (i.e. ≤12 months, short disease duration group).

RESULTS

CSF levels of all biomarkers were similar in NT1 subjects and controls. The comparison between NT1 with short and long disease duration only revealed slightly higher levels of CSF amyloid-beta 1-40 in the former group (median 9,549.5, interquartile range [IQR] 7,064.2-11,525.0 vs. 6,870.0, IQR 5,133.7-9,951.2, p = 0.043). CSF storage time did not influence the levels of the tested biomarkers.

CONCLUSIONS

The measurement of CSF total-tau, phosphorylated-tau, amyloid-beta 1-40 and 1-42, and NfL proteins is not informative in NT1.

摘要

研究目的

测量不同病程的 1 型发作性睡病（NT1）患者脑脊液（CSF）中 5 种神经退行性生物标志物的水平。

方法

按照 CSF 采集和储存的标准化方案，我们测量了 30 名非神经科对照者和 36 名 NT1 患者（包括 14 名疾病近期发作患者（即≤12 个月，病程短组））的 CSF 总tau 和磷酸化tau、β淀粉样蛋白 1-40 和 1-42 以及神经丝轻链（NfL）蛋白。

结果

NT1 患者和对照组的 CSF 各标志物水平相似。NT1 患者中病程短和长组之间的比较仅显示前者 CSF 中β淀粉样蛋白 1-40 水平略高（中位数分别为 9549.5、7064.2-11525.0 和 6870.0、5133.7-9951.2，p=0.043）。CSF 储存时间对检测标志物水平没有影响。