Effect of low-frequency ultrasound on the particle size, solubility and surface charge of reconstituted sodium caseinate.

Low-frequency sonication (20 kHz) was applied to sodium caseinate suspensions (4%, 7% and 10% protein concentrations) at pH 4.0, 4.6, 6.7 and 9.0. Particle size, zeta potential and solubility analysis were used to evaluate the physical changes of the sodium caseinate suspensions before and after the application of ultrasound. At pH 6.7 the particle size remained between 5 and 7 µm for all concentrations before and after sonication (15-400 J/mL), resulting in no significant change (p > 0.05). Similarly, sonication did not significantly (p > 0.05) affect the solubility at pH 6.7. At this pH, the initial solubility was high at 94-98% (w/w) before sonication. At pH 9.0 for 4% and 7% concentrations, suspensions became more negatively charged and the initial particle size increased to 78-82 µm. In the presence of larger suspensions, the application of ≥15 J/mL reduced the particle size to less than 2 µm. By contrast to pH 6.7, the solubility at pH 9.0 for 4% and 7% protein suspensions reached 99% before and after sonication. Viscosity was the highest (80 mPa.s at 15 sec) for a 10% protein concentration at pH 9.0. As the protein concentration of the sodium caseinate suspensions decreased from 10% to 4% at pH 9.0, the viscosity of the suspensions also decreased. However, application of low-frequency ultrasound had no effect on the viscosity of the sodium caseinate suspensions. Due to the absence of large insoluble aggregates in reconstituted sodium caseinate suspensions, the overall effect of low-frequency sonication were largely insignificant at native pH and only became evident at outlier pH values when the casein proteins associate.