Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, People's Republic of China.
J Biomater Sci Polym Ed. 2020 Jan;31(1):53-71. doi: 10.1080/09205063.2019.1675224. Epub 2019 Oct 16.
In this study, a novel magnetic solid phase extraction adsorbent was prepared, namely FeO@SiO as the carrier, quercetin as the template molecule, acrylamide (AM) as the functional monomer, ethylene glycol dimethacrylamide (EGDMA) as the crosslinker, 2,2'-azoisobutyronitrile (AIBN) as the initiator to prepare magnetic molecularly imprinted polymer (MMIPs) with specific adsorption of quercetin by surface molecular imprinting technique. MMIPs were characterized by Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD) and transmission electron microscope (TEM). The binding properties of MMIPs were evaluated by isothermal adsorption, kinetic adsorption and selective adsorption experiments. The results showed that the saturated adsorption capacity and adsorption time were 17.9 μmol/g and 20 min, respectively, and showed high selectivity for quercetin. Under optimized conditions, the limit of detection (LOD) and limit of quantitation (LOQ) were 55.327 ng/mL and 184.413 ng/mL, respectively. The recovery and RSD were 85.34-95.67% and 3.36-6.59, respectively. We successfully combined magnetic solid phase extraction with high performance liquid chromatography (MSPE-HPLC-UV) for the enrichment, separation and detection of quercetin in rat plasma. The results showed that the peak and half-life of quercetin in plasma were 1.5 h (32.310 μg/mL) and 3 h, respectively. The method was simple, rapid and efficient, and could be applied to the separation and detection of quercetin in complex matrices, and also provides a basis for the separation and identification of other natural chemical components.
在这项研究中，制备了一种新型的磁性固相萃取吸附剂，即 FeO@SiO2 作为载体，槲皮素作为模板分子，丙烯酰胺（AM）作为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）作为交联剂，2,2'-偶氮二异丁腈（AIBN）作为引发剂，通过表面分子印迹技术制备了对槲皮素有特异性吸附的磁性分子印迹聚合物（MMIPs）。通过傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和透射电子显微镜（TEM）对 MMIPs 进行了表征。通过等温吸附、动力学吸附和选择性吸附实验评价了 MMIPs 的结合性能。结果表明，饱和吸附容量和吸附时间分别为 17.9 μmol/g 和 20 min，对槲皮素有较高的选择性。在优化条件下，检测限（LOD）和定量限（LOQ）分别为 55.327 ng/mL 和 184.413 ng/mL。回收率和 RSD 分别为 85.34%-95.67%和 3.36%-6.59%。我们成功地将磁性固相萃取与高效液相色谱（MSPE-HPLC-UV）相结合，用于富集中、分离和检测大鼠血浆中的槲皮素。结果表明，槲皮素在血浆中的峰和半衰期分别为 1.5 h（32.310 μg/mL）和 3 h。该方法简单、快速、高效，可用于复杂基质中槲皮素的分离和检测，也为其他天然化学成分的分离和鉴定提供了依据。
