Pathologic Changes Associated With Respiratory Compromise And Morbidity Due To Massive Interlamellar Infection In Channel × Blue Hybrid Catfish.

There are multiple spp. (Myxozoa: Myxobolidae) endemic to North American catfish aquaculture that affect the gills of channel catfish and their hybrids. These parasites are morphologically similar, and confusion exists regarding the predilection sites and pathologic changes associated with different species. In the spring of 2018, channel () female × blue () male hybrid catfish from 2 separate commercial operations in northwest Mississippi were submitted for diagnostic assessment in response to observed morbidity and reduced feeding activity. Fish presented with unusually heavy infections of spp. plasmodia in the gills. The majority of gill filaments contained widespread, pinpoint, raised, white nodules corresponding microscopically to myxospore-filled plasmodia that obliterated interlamellar spaces. The bipolar myxospores were consistent with spp. described from North American ictalurids, possessing slender fusiform spore bodies and elongate bifurcate caudal processes. Associated microscopic lesions included lamellar fusion, epithelial hyperplasia, infrequent, localized, granulomatous branchitis, and rare cartilage lysis, suggesting impaired gill function. Mature plasmodia were excised by laser capture microdissection from ethanol-fixed, hematoxylin and eosin-stained histologic sections for molecular analysis. Fragments (700 bp) of a highly variable region of the gene, diagnostic for the Myxobolidae, were 100% similar at the nucleotide level to . Although mortality was negligible, fish in the affected ponds exhibited signs of respiratory distress similar to proliferative gill disease (PGD) caused by in channel and hybrid catfish. However, gross and microscopic lesions differed markedly from PGD, known colloquially as "hamburger gill disease." While has been reported from channel catfish, it is not typically associated with morbidity and mortality and has not previously been reported from channel × blue catfish hybrids. This work characterizes lesions and confirms the etiology of gill disease induced by the myxozoan . In addition to PGD and other non-parasitic conditions, massive interlamellar infection should be a differential consideration in pond-raised channel and hybrid catfish experiencing signs of respiratory distress.