de Sousa Barbosa Brenna, Rodrigues Silva Herlon Victor, Evaristo de Almeida Tabosa Beatriz, Gothardo Pereira Nunes Thalles, de Magalhães Francisco Felipe, Daniel Machado da Silva Lúcia
School of Veterinary Medicine, State University of Ceará (UECE), Fortaleza, Brazil.
Reprod Domest Anim. 2019 Dec;54(12):1630-1636. doi: 10.1111/rda.13572. Epub 2019 Oct 20.
Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morphology, were quantified the percentage of normal sperm cells, morphological changes of head, midpiece and tail. In morphometry, each normal sperm cell was measured for length, width, area and perimeter of head and midpiece, tail length and total length. The parameters ellipticity, elongation, regularity and rugosity were also determined. The percentage of normal sperm was 67.21%. Of the abnormalities, the curled/folded tail, followed by the curved midpiece, abnormal shaped head and detached head were the most quantified. The sperm head presented 5.56 ± 0.01 μm and 3.10 ± 0.01 μm of length and width, respectively. The head area was 16.94 ± 0.05 μm , while the perimeter was 16.16 ± 0.03 μm. In the derived parameters, the values were as follows: ellipticity of 1.81 ± 0.00; elongation of 21.39 ± 0.12; regularity of 0.81 ± 0.00; and rugosity of 0.14 ± 0.00. The midpiece presented length and width of 7.96 ± 0.01 μm and 0.76 ± 0.01 μm, respectively. The mean length of the sperm tail was 45.12 ± 0.06 μm, and the total cell size was 58.67 ± 0.06 μm. Thus, it was concluded that the cat sperm is an elongated cell, with high rugosity and regularity. The spermatic tail represents more than ¾ of the total length of the cell and the midpiece exceeds the length of the head.
精子形态测定法是一种通过精确测量配子及其结构的尺寸，使形态学评估具有客观性的工具。因此，本研究的目的是对家猫精子进行形态计量学特征分析。为此，在37℃下，从20对附睾中采集精子样本，并保存在TRIS稀释液中。取一份样本用孟加拉玫瑰红溶液制作涂片，随后分析其形态和形态计量学特征。在形态学方面，对正常精子细胞的百分比、头部、中段和尾部的形态变化进行了量化。在形态计量学方面，对每个正常精子细胞的头部和中段的长度、宽度、面积和周长、尾部长度以及总长度进行了测量。还确定了椭圆率、伸长率、规则度和粗糙度等参数。正常精子的百分比为67.21%。在异常情况中，卷曲/折叠尾出现的数量最多，其次是弯曲的中段、头部形状异常和头部脱落。精子头部的长度和宽度分别为5.56±0.01μm和3.10±0.01μm。头部面积为16.94±0.05μm²，周长为16.16±0.03μm。在导出参数方面，数值如下：椭圆率为1.81±0.00；伸长率为21.39±0.12；规则度为0.81±0.00；粗糙度为0.14±0.00。中段的长度和宽度分别为7.96±0.01μm和0.76±0.01μm。精子尾部的平均长度为45.12±0.06μm，细胞总大小为58.67±0.06μm。因此，得出的结论是，猫精子是一种细长的细胞，具有较高的粗糙度和规则度。精子尾部占细胞总长度的四分之三以上，中段超过头部的长度。
