Gunn 大鼠早产儿高胆红素血症模型。
A Gunn rat model of preterm hyperbilirubinemia.
机构信息
Division of Neonatology, Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, USA.
出版信息
Pediatr Res. 2020 Feb;87(3):480-484. doi: 10.1038/s41390-019-0599-x. Epub 2019 Oct 2.
BACKGROUND
The impact of bilirubin in preterm infants is poorly understood. An animal model would assist in improving understanding. The Gunn rat lacks uridine diphosphate-glucuronylsyl transferase 1 and can be made acutely hyperbilirubinemic by injection of sulfodimethoxine (sulfa), a drug that displaces bilirubin from albumin and thus increases free bilirubin.
METHODS
On postnatal day (P) 5, Gunn rats either heterozygous (Nj) or homozygous (jj) for glucuronosyltransferase activity were injected with either saline or sulfa. Behavior and cerebellar weight were measured.
RESULTS
Pups did not show any signs of acute bilirubin encephalopathy. Pup weight dropped significantly on P8 only in the jj-sulfa group. Behavior was affected only in the jj-sulfa group. Cerebellar weight was significantly less in the jj-sulfa group.
CONCLUSION
The Gunn rat pup model may be a good model to study hyperbilirubinemia in preterm infants.
背景
胆红素对早产儿的影响尚未被充分认识。动物模型将有助于提高对此的理解。Gunn 大鼠缺乏尿苷二磷酸葡萄糖醛酸基转移酶 1，可通过注射磺胺二甲氧嘧啶（磺胺）使其急性高胆红素血症，磺胺可将胆红素从白蛋白中置换出来，从而增加游离胆红素。
方法
在出生后第 5 天（P），Gunn 大鼠葡萄糖醛酸基转移酶活性为杂合子（Nj）或纯合子（jj），分别注射生理盐水或磺胺。测量行为和小脑重量。
结果
幼鼠没有出现任何急性胆红素脑病的迹象。仅在 jj-磺胺组中，幼鼠体重在 P8 时显著下降。只有在 jj-磺胺组中，行为才受到影响。jj-磺胺组的小脑重量明显减轻。
结论
Gunn 大鼠幼鼠模型可能是研究早产儿高胆红素血症的良好模型。
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