通过实时定量PCR早期检测真菌植物病原体：以松树皮焦病菌为例
Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine.
作者信息
Luchi Nicola, Santini Alberto, Salvianti Francesca, Pinzani Pamela
机构信息
Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Florence, Italy.
Molecular and Clinical Biochemistry Unit, Department of Experimental and Clinical Biomedical Sciences "Mario Serio", Careggi Hospital, University of Florence, Florence, Italy.
出版信息
Methods Mol Biol. 2020;2065:95-104. doi: 10.1007/978-1-4939-9833-3_8.
This chapter reports the use of real-time quantitative PCR to detect Diplodia sapinea, a fungal plant pathogen that causes shoot tip dieback and tree mortality on pine trees. This molecular approach represents a reliable and sensitive tool to detect fungal pathogens in DNA extracted from plant tissues and its use can be also recommended to study fungal behavior in host tissues by quantifying fungal growth in the latent phase, when symptoms in the host are not present yet.
本章报道了利用实时定量PCR检测松色二孢菌的方法，松色二孢菌是一种真菌性植物病原体，可导致松树梢枯和树木死亡。这种分子方法是一种可靠且灵敏的工具，可用于检测从植物组织中提取的DNA中的真菌病原体，并且还推荐通过在宿主尚未出现症状的潜伏期对真菌生长进行定量分析，来研究真菌在宿主组织中的行为。
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