评估大麻二酚对伯基特淋巴瘤免疫疗法的影响。
The evaluation of Cannabidiol's effect on the immunotherapy of Burkitt lymphoma.
机构信息
Division of Blood and Bone Marrow Transplantation, Department of Medicine, University of Louisville School of Medicine, Louisville, KY, USA; Division of Haematology, National Center for Global Health and Medicine, Tokyo, Japan.
Dupont Manual High School, Louisville, KY, USA.
出版信息
Biochem Biophys Res Commun. 2019 Nov 26;520(1):225-230. doi: 10.1016/j.bbrc.2019.10.001. Epub 2019 Oct 3.
AIM
AF1q has a precise oncogenic function. The purpose of this study is to investigate whether CBD has an effect on the AF1q/ICAM-1 regulatory axis in Burkitt's lymphoma (BL), and thus has potential to enhance immunotherapy and reduce side effects.
METHODS
We established BL cell lines with altered AF1q expression using lentivirus. After confirmation of gene expression by RT-PCR, cells were treated with CBD followed by co-culture of killing assay.
RESULTS
AF1q increased oncogenic growth and colony formation, and induced resistance against cell-mediated cytotoxic chemotherapy through attenuation of ICAM-1 expression in BL. CBD was able to reverse the acquired resistance mediated by AF1q/ICAM-1 regulatory axis.
CONCLUSION
CBD holds potential to enhance the efficacy of immunotherapy for BL with hyperactive AF1q/ICAM-1 regulatory axis, and warrants further study.
目的
AF1q 具有精确的致癌功能。本研究旨在探讨 CBD 是否对 Burkitt 淋巴瘤（BL）中的 AF1q/ICAM-1 调节轴有影响，从而有可能增强免疫疗法并减少副作用。
方法
我们使用慢病毒建立了 AF1q 表达改变的 BL 细胞系。通过 RT-PCR 确认基因表达后，用 CBD 处理细胞，然后进行杀伤测定的共培养。
结果
AF1q 通过下调 ICAM-1 的表达，增加了 BL 的致癌生长和集落形成，并诱导了对细胞介导的细胞毒性化疗的耐药性。CBD 能够逆转由 AF1q/ICAM-1 调节轴介导的获得性耐药性。
结论
CBD 有可能增强针对具有过度活跃的 AF1q/ICAM-1 调节轴的 BL 的免疫疗法的疗效，值得进一步研究。
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