Baek Seungik, Liu Chun, Gou Kun, Kim Jungsil, Gharahi Hamidreza, Chan Christina
Department of Mechanical Engineering, 428 S. Shaw Lane, Michigan State University, East Lansing, MI 48824, US, Tel.: +1-517-432-3161.
Department of Chemical Engineering, Michigan State University, East Lansing, MI 48824, US.
J Elast. 2019 Aug;136(2):137-157. Epub 2018 Sep 25.
Recent studies suggest that cells routinely probe their mechanical environments and that this mechanosensitive behavior regulates some of their cellular activities. The finite elasticity theory of small-on-large deformation (SoL) has been shown to be effective in interpreting the mechanosensitive behavior of cells on a substrate that has been subjected to a prior large static stretch before the culturing of the cells. Small on large deformation is the superposition of a small deformation onto a prior large deformation that serves as the new reference configuration. This article aims to refine SoL theory to develop a theoretical framework for improved physical interpretation of mechanosensing. Given the initial large deformation in SoL, the stress generated by the small deformation is linearized, and the linearized elasticity tensor is taken to be a significant factor facilitating prediction of cellular behavior. The pre-stretch is shown to produce direction-based, effective elastic moduli for cellular mechanosensing. The utility of this SoL theory is illustrated by culturing of two different cell types grown on uniaxially pre-stretched surfaces that induce changes to the cell orientation and behavior.
最近的研究表明，细胞会常规性地探测其力学环境，并且这种机械敏感行为会调节它们的一些细胞活动。小变形叠加在大变形上（SoL）的有限弹性理论已被证明在解释细胞在培养前已受到先前大静态拉伸的基底上的机械敏感行为方面是有效的。小变形叠加在大变形上是指将小变形叠加到先前的大变形上，该大变形用作新的参考构型。本文旨在完善SoL理论，以建立一个用于改进机械传感物理解释的理论框架。考虑到SoL中的初始大变形，由小变形产生的应力被线性化，并且线性化的弹性张量被视为有助于预测细胞行为的一个重要因素。预拉伸被证明会为细胞机械传感产生基于方向的有效弹性模量。通过培养在单轴预拉伸表面上生长的两种不同细胞类型来说明这种SoL理论的实用性，这种预拉伸表面会引起细胞取向和行为的变化。
