Vidal-Ingigliardi D, Raibaud O
Nucleic Acids Res. 1985 Aug 26;13(16):5919-26. doi: 10.1093/nar/13.16.5919.
We describe a technique which allows one to insert any promoter in front of the chromosomal malPQ operon. This can be done easily by using only one plasmid, one strain, and two simple selections. Properties of the final chromosomal fusion are such that the level of amylomaltase, the product of the malQ gene, measures quantitatively the efficiency of the inserted promoter. This method was utilized to compare the efficiency of four well-known promoters: lacZp, trp, tac, lambdaPR and three malT activated promoters: malPp, malkP and malEp.
我们描述了一种技术,该技术可使人们在染色体malPQ操纵子前插入任何启动子。仅使用一个质粒、一个菌株和两个简单的筛选就能轻松做到这一点。最终染色体融合体的特性是,malQ基因的产物淀粉麦芽糖酶的水平可定量测量插入启动子的效率。该方法用于比较四个著名启动子:lacZp、trp、tac、λPR,以及三个malT激活的启动子:malPp、malkP和malEp的效率。