卵丘细胞是细胞治疗的潜在候选者。
Cumulus Cells Are Potential Candidates for Cell Therapy.
机构信息
Department of Biochemistry, Yildiz Technical University, Istanbul, Turkey.
Division of Pediatric Hematology/Oncology, Health Science University Kartal Lutfi Kirdar Education and Research Hospital, Istanbul, Turkey.
出版信息
In Vivo. 2019 Nov-Dec;33(6):1921-1927. doi: 10.21873/invivo.11686.
BACKGROUND/AIM: Cumulus cells (CCs) originate from the membrane granulosa cells and surround oocytes during follicle maturation. CCs produce high levels of hyaluronan that targets CD44, which is a major tumorigenic marker. This study aimed to investigate whether CCs have a role in cell therapy by targeting CD44 in pancreatic cancer cells.
MATERIALS AND METHODS
CCs were isolated from the oocytes and incubated in a hypoxic environment. BxPC-3 pancreatic cancer cells were treated with CC conditioned media for three days.
RESULTS
Conditioned media of CC cells incubated in hypoxic conditions caused a 25% reduction in the viability of BxPC-3 cells. Expression of anti-apoptotic genes was down-regulated, while that of pro-apoptotic genes was upregulated. An increased number of BxPC-3 cells exhibited increased levels of reactive oxygen species and arrested in the synthesis (S) phase of the cell cycle.
CONCLUSION
CCs conditioned medium induced apoptosis of pancreatic cancer cells.
背景/目的：卵丘细胞（cumulus cells，CCs）起源于颗粒细胞层，在卵泡成熟过程中围绕卵母细胞。CCs 产生高水平的透明质酸，其靶向 CD44，后者是主要的致瘤标志物。本研究旨在通过靶向胰腺癌细胞上的 CD44 来探讨 CCs 在细胞治疗中的作用。
材料和方法
从卵母细胞中分离 CCs，并在低氧环境中孵育。用 CC 条件培养基处理 BxPC-3 胰腺癌细胞 3 天。
结果
在低氧条件下孵育的 CC 细胞的条件培养基使 BxPC-3 细胞的活力降低了 25%。抗凋亡基因的表达下调，而促凋亡基因的表达上调。越来越多的 BxPC-3 细胞表现出更高水平的活性氧，并在细胞周期的合成（S）期停滞。
结论
CC 条件培养基诱导胰腺癌细胞凋亡。
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