Kulikov V I, Dmitrieva L N, Kashkin K P
Biokhimiia. 1988 May;53(5):786-92.
The interaction of liposomes derived from total lipids of mouse spleen and liver with mouse spleen cells was studied. It was shown that the binding of these liposomes is much higher than the binding of liposomes obtained from a model lipid mixture--phosphatidylcholine--phosphatidylethanolamine--cholesterol (2:1:1). Adherent and nonadherent spleen cells were found to have affinity for liposomes derived from total lipids of spleen or liver. Removal of gangliosides and protein contaminants from the liposomes derived from total spleen lipids caused an increased binding of liposomes to spleen cells. Multilamellar liposomes bound more effectively to ultrasonicated vesicles having a homologous lipid composition than the liposomes with a different lipid composition. The increased affinity of liposomes derived from total lipids of spleen or liver for spleen cells may account for the identical fluidity of the lipid bilayer of liposomes and plasma membranes of spleen cells.
研究了从小鼠脾脏和肝脏的总脂质衍生的脂质体与小鼠脾脏细胞的相互作用。结果表明，这些脂质体的结合力远高于从模型脂质混合物——磷脂酰胆碱——磷脂酰乙醇胺——胆固醇（2:1:1）获得的脂质体的结合力。发现贴壁和非贴壁脾脏细胞对源自脾脏或肝脏总脂质的脂质体具有亲和力。从脾脏总脂质衍生的脂质体中去除神经节苷脂和蛋白质污染物会导致脂质体与脾脏细胞的结合增加。多层脂质体比具有不同脂质组成的脂质体更有效地结合具有同源脂质组成的超声处理囊泡。源自脾脏或肝脏总脂质的脂质体对脾脏细胞亲和力的增加可能解释了脂质体脂质双层与脾脏细胞质膜相同的流动性。
