IRTA, Ctra. Poble Nou, km. 5.5, 43540 Sant Carles de la Ràpita, Tarragona Spain; Centre of Marine Sciences (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro Portugal.
Institute of Aquaculture, University of Stirling, FK9 4LU Stirling Scotland UK.
Physiol Behav. 2020 Jan 1;213:112724. doi: 10.1016/j.physbeh.2019.112724. Epub 2019 Nov 1.
In fish, proactive and reactive individual stress copying styles (SCS) have been used to resolve variation in molecular expression data. Stress coping styles have been previously described in several stages of Solea senegalensis by validating for the species the use of standard behavioural screening tests. The present study aimed to link behavioural SCS tests with brain transcript abundance in early Senegalese sole juveniles in order to observe the natural variation in a molecular pathway in this species. A total of 50 juveniles were subjected to three individual behavioural (Restraining, New environment and Confinement) and one group (Risk-taking) screening tests. The fish were classified in SCS categories by applying a hierarchical cluster to the variable "Total activity" (the total activity time that the fish was moving in each individual test). Three categories were defined, proactive, intermediate and reactive sole. Six transcripts were chosen and tested, one related to basic metabolism (gapdh-2), three to feeding behaviour (per1, igf-Ia, pparß) and two to the stress response (crh-BP and hsp90aa) in 30 juveniles (10 individuals per SCS category) using rt-qPCR to observe differences in the abundance of those transcripts among SCS. Four transcripts were differentially expressed (DETs) among them. The transcript gapdh-2 showed up-regulation for proactive and intermediate SCS sole while reactive individuals showed down-regulation. Target mRNAs per1, igf-Ia and pparß, showed different levels of up-regulation for proactive and reactive fish while intermediates were highly down-regulated. Surprisingly no differences in stress related transcripts were observed. Correlations were found between variation in coping styles and variation in the abundance of mRNAs involved in important biological functions in Senegalese sole. These results are the first evidence of the relationship between the behavioural individual variation and the fluctuation in brain transcripts abundance in Senegalese sole.
在鱼类中，已经使用主动和被动个体应激复制风格（SCS）来解决分子表达数据的变化。在塞内加尔鳎的几个阶段，通过验证该物种对标准行为筛选测试的使用，已经描述了应激应对风格。本研究旨在将行为 SCS 测试与早期塞内加尔鳎幼鱼的大脑转录丰度联系起来，以观察该物种中分子途径的自然变化。总共对 50 条幼鱼进行了三种个体行为（约束、新环境和禁闭）和一种群体（冒险）筛选测试。通过对“总活动”（鱼在每个个体测试中移动的总活动时间）这个变量应用层次聚类，将鱼分类为 SCS 类别。定义了三个类别，主动、中间和被动鳎。选择并测试了 6 个转录本，一个与基础代谢有关（gapdh-2），三个与摄食行为有关（per1、igf-Ia、pparß），两个与应激反应有关（crh-BP 和 hsp90aa），在 30 条幼鱼（10 条个体/ SCS 类别）中使用 rt-qPCR 观察这些转录本在 SCS 中的丰度差异。其中 4 个转录本表现出差异表达（DETs）。转录本 gapdh-2 表现出主动和中间 SCS 鳎的上调，而反应性个体表现出下调。靶标 mRNAs per1、igf-Ia 和 pparß 表现出主动和反应性鱼类的不同上调水平，而中间物则高度下调。令人惊讶的是，没有观察到与应激相关的转录本的差异。在鳎的应激方式变化与大脑中与重要生物学功能相关的 mRNA 丰度变化之间发现了相关性。这些结果首次证明了塞内加尔鳎的行为个体变异与大脑转录丰度波动之间的关系。
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