一种用于从支气管扩张症患者呼吸道标本中分离复合菌的新型快速生长分枝杆菌培养基的评估。
Evaluation of a novel rapidly-growing mycobacteria medium for isolation of complex from respiratory specimens from patients with bronchiectasis.
作者信息
Brown-Elliott Barbara A, Molina Susan, Fly Travis, Njie Ousman, Stribley Patricia, Stephenson Dominic, Wallace Richard J, Perry John D
机构信息
Mycobacteria/Nocardia Research Laboratory, The University of Texas Health Science Center at Tyler, Texas, United States.
Pathology Laboratory, The University of Texas Health Science Center at Tyler, Texas, United States.
出版信息
Heliyon. 2019 Oct 24;5(10):e02684. doi: 10.1016/j.heliyon.2019.e02684. eCollection 2019 Oct.
This single center study assessed the performance of a novel solid rapidly-growing mycobacteria (RGM) medium for the recovery of nontuberculous mycobacteria (NTM), especially complex, in patients with underlying bronchiectasis. A total of 297 mycobacterial sputa from 116 patients were plated directly on RGM medium and, following decontamination, onto an agar biplate [Middlebrook 7H11 and Mitchison (selective) agar] and into broth media (VersaTrek). The recovery of complex was increased by approximately 12% by implementation of the RGM medium. Contamination was reduced to 2% from 48% and 95% on routine solid media and broth cultures respectively. Our study corroborated previous studies in that recovery of complex was enhanced and contamination was virtually eliminated without the need for specimen decontamination when utilizing RGM medium.
这项单中心研究评估了一种新型固体快速生长分枝杆菌（RGM）培养基在潜在支气管扩张症患者中用于分离非结核分枝杆菌（NTM），尤其是复合群的性能。来自116例患者的总共297份分枝杆菌痰标本直接接种于RGM培养基上，经去污处理后，接种于琼脂双平板[Middlebrook 7H11和米切森（选择性）琼脂]及肉汤培养基（VersaTrek）中。使用RGM培养基使复合群的分离率提高了约12%。污染率从常规固体培养基和肉汤培养的分别48%和95%降至2%。我们的研究证实了之前的研究，即使用RGM培养基时，复合群的分离率提高且几乎消除了污染，无需进行标本去污处理。
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