Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Hospital of Jiangnan University (The Fourth People's Hospital of Wuxi), Wuxi, China.
Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):8897-8904. doi: 10.26355/eurrev_201910_19285.
Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors worldwide. Recent studies have revealed that long non-coding RNAs (lncRNAs) play important roles in the progression of tumorigenesis. The aim of this study was to identify the exact role of lncRNA CASC15 in the progression of NPC.
CASC15 expression in both 54 paired NPC patients' tissue samples and cell lines was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Moreover, the function of CASC15 was identified by performing cell proliferation assay, transwell assay and wound healing assay in vitro. The underlying mechanism was explored through Luciferase assay and RT-qPCR. In addition, tumor formation and metastasis assays were conducted in vivo.
CASC15 expression in NPC tissues was markedly higher than that of adjacent non-tumor tissues. The proliferation, migration and invasion of NPC cells were significantly inhibited after knockdown of CASC15 in vitro. Our further experiments revealed that miR-101-3p was remarkably up-regulated via knockdown of CASC15. Meanwhile, miR-101-3p was a direct target of CASC15 in NPC. Furthermore, tumor formation and metastasis of NPC were significantly inhibited via knockdown of CASC15 in nude mice.
CASC15 enhances NPC cell proliferation and metastasis via sponging miR-101-3p in vitro and in vivo.
鼻咽癌（NPC）是全球最常见的恶性肿瘤之一。最近的研究表明，长链非编码 RNA（lncRNA）在肿瘤发生发展中发挥重要作用。本研究旨在确定 lncRNA CASC15 在 NPC 进展中的确切作用。
通过实时定量聚合酶链反应（RT-qPCR）检测 54 对 NPC 患者组织样本和细胞系中的 CASC15 表达。此外，通过体外细胞增殖试验、Transwell 试验和划痕愈合试验来鉴定 CASC15 的功能。通过荧光素酶报告基因检测和 RT-qPCR 探讨其潜在机制。此外，还进行了体内肿瘤形成和转移试验。
NPC 组织中的 CASC15 表达明显高于相邻非肿瘤组织。体外敲低 CASC15 后，NPC 细胞的增殖、迁移和侵袭明显受到抑制。我们的进一步实验表明，miR-101-3p 通过敲低 CASC15 而显著上调。同时，miR-101-3p 是 NPC 中 CASC15 的直接靶标。此外，在裸鼠中敲低 CASC15 可显著抑制 NPC 的肿瘤形成和转移。
CASC15 通过体内外海绵吸附 miR-101-3p 增强 NPC 细胞的增殖和转移。
