School of Aerospace Medicine, Fourth Military Medical University, Xi'an, China.
Department of Ultrasound Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
Am J Physiol Heart Circ Physiol. 2019 Dec 1;317(6):H1376-H1387. doi: 10.1152/ajpheart.00382.2019. Epub 2019 Nov 8.
There is still lack of a simple, accurate, and noninvasive method for rat aortic pulse wave velocity (PWV) measurement, especially the transit distance cannot be accurately measured. Thus, we aimed to derive an equation for aortic transit distance as a function of the nose-to-rump length () and to test the hypothesis that aortic PWV measured by new equation combined with Doppler ultrasound (the "equation method") may have stronger correlation with invasive measurements than traditional "body surface method." Two-hundred male Sprague-Dawley (SD) rats (age ranged 5-24 wk) were included in , and the aortic transit distances were measured postmortem. In , heart-femoral PWV and carotid-femoral PWV were measured by equation method (hfPWV, cfPWV) and also by traditional body surface method (hfPWV, cfPWV) in another 30 young and 28 old rats. These measurements were then validated against invasively measured hfPWV and cfPWV from the same animal. showed that the heart-femoral transit distance could be calculated by 0.6086 × - 1.6523, and the carotid-femoral transit distance by 0.4614 × L + 1.8335. In , in young rats, the Pearson between hfPWV, cfPWV, hfPWV, and cfPWV and their corresponding invasive measurement were 0.8962, 0.8509, 0.8387, and 0.7828, respectively (all < 0.0001). In the old group, the results were 0.8718, 0.7999, 0.8330, and 0.7112, respectively (all < 0.0001). The hfPWV and cfPWV showed better agreement with hfPWV and cfPWV and lower intra- and interobserver variability compared with hfPWV and cfPWV in both groups. These findings demonstrate that this novel methodology provides a simple and reliable method for rat noninvasive aortic PWV measurement. First, when measuring aortic PWV in SD rat models, the heart-femoral transit distance can be estimated by 0.6086 × - 1.6523, and the carotid-femoral distance transit distance can be estimated by 0.4614 × + 1.8335, where (in mm) is nose-to-rump length. Second, this novel methodology for aortic PWV measurement was validated with a closer correlation with the invasive measurements than traditional approach in young and old rats. Third, this study provides a simple and reliable method for rat noninvasive aortic PWV measurement.
目前，仍然缺乏一种简单、准确、非侵入性的方法来测量大鼠的主动脉脉搏波速度（PWV），尤其是无法准确测量传输距离。因此，我们旨在推导出一个主动脉传输距离的方程，作为鼻到尾部长度（）的函数，并检验以下假设：通过新方程结合多普勒超声测量的主动脉 PWV（“方程法”）与传统的“体表面积法”相比，可能与侵入性测量具有更强的相关性。纳入了 200 只雄性 Sprague-Dawley（SD）大鼠（年龄范围为 5-24 周），并在死后测量主动脉传输距离。在另一项研究中，又有 30 只年轻大鼠和 28 只老年大鼠分别通过方程法（hfPWV、cfPWV）和传统体表面积法（hfPWV、cfPWV）测量心-股 PWV 和颈-股 PWV，这些测量结果与同一动物的侵入性测量 hfPWV 和 cfPWV 进行了验证。结果表明，心-股传输距离可通过 0.6086×-1.6523 计算，颈-股传输距离可通过 0.4614×L+1.8335 计算。在年轻大鼠中，hfPWV、cfPWV、hfPWV 和 cfPWV 与其相应的侵入性测量之间的 Pearson 相关系数分别为 0.8962、0.8509、0.8387 和 0.7828（均 ＜0.0001）。在老年大鼠组中，结果分别为 0.8718、0.7999、0.8330 和 0.7112（均 ＜0.0001）。与 hfPWV 和 cfPWV 相比，hfPWV 和 cfPWV 与 hfPWV 和 cfPWV 具有更好的一致性，并且在两组中均具有较低的观察者内和观察者间变异性。这些发现表明，这种新方法为大鼠的非侵入性主动脉 PWV 测量提供了一种简单可靠的方法。首先，在测量 SD 大鼠模型的主动脉 PWV 时，心-股传输距离可以通过 0.6086×-1.6523 来估算，颈-股传输距离可以通过 0.4614×+1.8335 来估算，其中（mm）为鼻到尾部长度。其次，与传统方法相比，这种新的主动脉 PWV 测量方法在年轻和老年大鼠中与侵入性测量具有更密切的相关性。第三，本研究为大鼠非侵入性主动脉 PWV 测量提供了一种简单可靠的方法。
