细胞生物学：从 HAC 中剔除 Alpha 卫星。

Cell Biology: Hacking Alpha Satellites out of the HAC.

机构信息

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA; Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.

出版信息

Curr Biol. 2019 Nov 18;29(22):R1194-R1196. doi: 10.1016/j.cub.2019.09.059.

DOI:10.1016/j.cub.2019.09.059

PMID:31743678

Abstract

Human artificial chromosomes (HACs) are a potentially powerful technique for genomic engineering, but their use is limited by the repetitive centromeric alpha-satellite DNA needed to form a centromere. A new study presents a method to induce HAC centromere formation on non-repetitive templates through sequence-directed CENP-A nucleosome seeding.

摘要

人类人工染色体 (HAC) 是一种用于基因组工程的潜在强大技术，但由于需要形成着丝粒的重复的着丝粒α卫星 DNA，其使用受到限制。一项新的研究提出了一种通过序列指导的 CENP-A 核小体播种在非重复模板上诱导 HAC 着丝粒形成的方法。