Johnson-Mackinnon Jessica C, Crosbie Philip B B, Karlsbakk Egil, Marcos-Lopez Mar, Paley Richard, Nowak Barbara F, Bridle Andrew R
University of Tasmania, Institute for Marine and Antarctic Studies, Locked Bag 1370 Launceston, Tasmania 7250, Australia.
Department of Biology, University of Bergen, Bergen, N5020, Norway.
Pathogens. 2019 Nov 19;8(4):244. doi: 10.3390/pathogens8040244.
is the aetiological agent of amoebic gill disease (AGD), a disease that affects farmed Atlantic salmon worldwide. Multilocus sequence typing (MLST) and Random Amplified Polymorphic DNA (RAPD) are PCR-based typing methods that allow for the highly reproducible genetic analysis of population structure within microbial species. To the best of our knowledge, this study represents the first use of these typing methods applied to with the objective of distinguishing geographical isolates. These analyses were applied to a total of 16 isolates from Australia, Canada, Ireland, Scotland, Norway, and the USA. All the samples from Australia came from farm sites on the island state of Tasmania. Genetic polymorphism among isolates was more evident from the RAPD analysis compared to the MLST that used conserved housekeeping genes. Both techniques consistently identified that isolates of from Tasmania, Australia were more similar to each other than to the isolates from other countries. While genetic differences were identified between geographical isolates, a BURST analysis provided no evidence of a founder genotype. This suggests that emerging outbreaks of AGD are not due to rapid translocation of this important salmonid pathogen from the same area.
是阿米巴鳃病（AGD）的病原体，AGD是一种影响全球养殖大西洋鲑鱼的疾病。多位点序列分型（MLST）和随机扩增多态性DNA（RAPD）是基于PCR的分型方法，可对微生物物种内的种群结构进行高度可重复的遗传分析。据我们所知，本研究首次使用这些分型方法来区分地理分离株。这些分析应用于来自澳大利亚、加拿大、爱尔兰、苏格兰、挪威和美国的总共16个分离株。来自澳大利亚的所有样本均来自塔斯马尼亚岛州的养殖场。与使用保守管家基因的MLST相比，RAPD分析中分离株之间的遗传多态性更为明显。两种技术都一致表明，来自澳大利亚塔斯马尼亚的分离株彼此之间比与其他国家的分离株更为相似。虽然在地理分离株之间发现了遗传差异，但BURST分析没有提供奠基者基因型的证据。这表明AGD的新爆发并非由于这种重要鲑科鱼类病原体从同一地区的快速转移所致。
