School of Chemical and Environmental Engineering, Jiangsu University of Technology, Changzhou, People's Republic of China.
Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, School of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou, People's Republic of China.
Appl Biochem Biotechnol. 2020 Apr;190(4):1289-1303. doi: 10.1007/s12010-019-03154-3. Epub 2019 Nov 21.
In this study, chemoenzymatic synthesis of furfuryl alcohol from biomass (e.g., corncob, bamboo shoot shell, and rice straw) was attempted by the tandem catalysis with Lewis acid (SnCl or solid acid SO/SnO-bentonite) and biocatalyst in one-pot manner. Compared with SnCl, solid acid SO/SnO-bentonite had higher catalytic activity for converting biomass into furfural, which could be biologically converted into furfuryl alcohol with Escherichia coli CCZU-H15 whole-cell harboring reductase activity. Sequential catalysis of biomass into furfural with SO/SnO-bentonite (3.0 wt%) at 170 °C for 0.5 h and bioreduction of furfural with whole cells at 30 °C for 4.5 h were used for the effective synthesis of furfuryl alcohol in one-pot media. Corncob, bamboo shoot shell, and rice straw (3.0 g, dry weight) could be converted into 65.7, 50.3, and 58.5 mM furfuryl alcohol with the yields of 0.26, 0.25, and 0.23 g furfuryl alcohol/(g xylan in biomass) in 40 mL reaction media. Finally, an efficient process of recycling and reusing of SO/SnO-bentonite catalyst and immobilized whole-cell biocatalyst was developed for the chemoenzymatic synthesis of furfuryl alcohol from biomass in the one-pot reaction system.
本研究尝试通过串联催化，在一锅法中使用路易斯酸（SnCl 或固体酸 SO/SnO-膨润土）和生物催化剂，从生物质（如玉米芯、竹笋壳和稻草）中化学酶法合成糠醇。与 SnCl 相比，固体酸 SO/SnO-膨润土对将生物质转化为糠醛具有更高的催化活性，而糠醛可以通过具有还原酶活性的大肠杆菌 CCZU-H15 全细胞生物转化为糠醇。在 170°C 下用 SO/SnO-膨润土（3.0wt%）对生物质进行 0.5 小时的串联催化，然后在 30°C 下用全细胞进行生物还原 4.5 小时，可用于在一锅介质中有效合成糠醇。在 40mL 反应介质中，3.0g（干重）玉米芯、竹笋壳和稻草可分别转化为 65.7、50.3 和 58.5mM 糠醇，糠醇的产率分别为 0.26、0.25 和 0.23g 糠醇/（生物质中木聚糖的 g 数）。最后，开发了一种有效的 SO/SnO-膨润土催化剂和固定化全细胞生物催化剂的循环再利用方法，用于在一锅反应体系中从生物质进行化学酶法合成糠醇。
