Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Eur J Immunol. 2020 Mar;50(3):353-362. doi: 10.1002/eji.201948342. Epub 2019 Dec 11.
Conditional mutagenesis and fate mapping have contributed considerably to our understanding of physiology and pathology. Specifically, Cre recombinase-based approaches allow the definition of cell type-specific contributions to disease development and of inter-cellular communication circuits in respective animal models. Here we compared Cx cr1 and Sall1 transgenic mice and their use to decipher the brain macrophage compartment as a showcase to discuss recent technological advances. Specifically, we highlight the need to define the accuracy of Cre recombinase expression, as well as strengths and pitfalls of these particular systems that should be taken into consideration when applying these models.
条件性突变和命运图谱分析极大地促进了我们对生理学和病理学的理解。具体来说，基于 Cre 重组酶的方法可以定义特定细胞类型对疾病发展的贡献，以及相应动物模型中细胞间通讯回路。在这里，我们比较了 Cxcr1 和 Sall1 转基因小鼠及其在揭示大脑巨噬细胞区室方面的应用，以此为例来讨论最近的技术进展。具体来说，我们强调需要定义 Cre 重组酶表达的准确性，以及在应用这些模型时应考虑这些特定系统的优缺点。
