微小 RNA-105 在食管癌中发挥独立的预后作用，并作为一种癌基因。
MicroRNA-105 plays an independent prognostic role in esophageal cancer and acts as an oncogene.
机构信息
Department of Oncology, Dongying People's Hospital of Shandong Province, Dongying, Shandong, China.
Department of Thoracic Surgery, 970 Hospital of the PLA Joint Logistic Support Force, Yantai, Shandong, China.
出版信息
Cancer Biomark. 2020;27(2):173-180. doi: 10.3233/CBM-180.
BACKGROUND
Esophageal cancer is a common tumor with high mortality worldwide. In the present study, we aimed to investigate the prognostic significance and regulatory effects of miR-105 on cellular functions of esophageal cancer cells.
METHODS
The expression level of miR-105 was analyzed in esophageal cancer tissues and cell lines by qRT-PCR. Survival analysis was carried out using the Kaplan-Meier and the prognostic significance of miR-105 was analyzed with Cox regression analysis. The effects of miR-105 on cell proliferation, migration, and invasion abilities were detected with cellular experiments.
RESULTS
We found that miR-105 was significantly upregulated in esophageal cancer tissues and cell lines, compared with the control group, respectively. Moreover, overexpression of miR-105 was significantly associated with positive lymph node metastasis, advanced TNM stage, and poor overall survival. In addition, overexpression of miR-105 promoted cell proliferation, migration, and invasion in esophageal cancer cells, while downregulation of miR-105 suppressed these cellular behaviors.
CONCLUSION
Our findings indicate that a higher level of miR-105 predicts poorer prognosis in esophageal cancer patients, and miR-105 can promote esophageal cancer cell proliferation, migration, and invasion.
背景
食管癌是一种常见的肿瘤，全球死亡率较高。本研究旨在探讨 miR-105 对食管癌细胞功能的预后意义和调控作用。
方法
通过 qRT-PCR 分析食管癌组织和细胞系中 miR-105 的表达水平。采用 Kaplan-Meier 进行生存分析，并采用 Cox 回归分析分析 miR-105 的预后意义。通过细胞实验检测 miR-105 对细胞增殖、迁移和侵袭能力的影响。
结果
我们发现，miR-105 在食管癌组织和细胞系中的表达明显高于对照组。此外，miR-105 的过表达与阳性淋巴结转移、晚期 TNM 分期和不良总生存期显著相关。此外，miR-105 的过表达促进了食管癌细胞的增殖、迁移和侵袭，而 miR-105 的下调则抑制了这些细胞行为。
结论
我们的研究结果表明，较高水平的 miR-105 预示着食管癌患者预后不良，miR-105 可促进食管癌细胞的增殖、迁移和侵袭。
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