Department of Biotechnology, Panjab University, Chandigarh, India.
Future Microbiol. 2019 Nov;14:1397-1415. doi: 10.2217/fmb-2019-0162. Epub 2019 Dec 4.
To elucidate the role of Rv2223c in . Purified recombinant Rv2223c protein was characterized. Expression of in the presence of different stress environment and subcellular localization were performed in H37Ra and (). Effect of its overexpression on growth rate, infection and intracellular survival in THP-1/PBMC cells were studied. rRv2223c demonstrated esterase activity with preference for NP-octanoate and hydrolyzed trioctanoate to di- and mono-octanoate. Expression of was upregulated in acidic and nutritive stress conditions. rRv2223c was identified in extracellular and cell wall fractions. exhibited enhanced growth, survival during stress, infection and intracellular survival. Rv2223c is a secretary, carboxyl-esterase, with enhanced expression under acidic and nutritive stress condition and might help in intracellular survival of bacteria.
为了阐明 Rv2223c 在. 中的作用。纯化了重组 Rv2223c 蛋白，并对其进行了表征。在不同的应激环境下表达 ，并在 H37Ra 和 () 中进行了亚细胞定位。研究了其过表达对 THP-1/PBMC 细胞生长速度、感染和细胞内存活的影响。rRv2223c 表现出酯酶活性，对 NP-辛酸酯有偏好，并将三辛酸酯水解为二辛酸酯和单辛酸酯。 在酸性和营养应激条件下表达上调。rRv2223c 存在于细胞外和细胞壁部分。 表现出增强的生长、应激期间的存活、感染和细胞内存活。 Rv2223c 是一种分泌型羧酸酯酶，在酸性和营养应激条件下表达增强，可能有助于细菌的细胞内存活。
