Department of Spine Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Zhongshan Road 321, Nanjing, 210008, China.
Eur Spine J. 2020 Apr;29(4):737-743. doi: 10.1007/s00586-019-06217-5. Epub 2019 Dec 12.
The study is aimed to investigate the expression of PAX3 in bilateral paravertebral muscles in adolescent idiopathic scoliosis (AIS) and controls, and to further clarify its association with the paravertebral muscle volume and curve severity.
Ten AIS patients and 10 age-matched controls with lumbar disc herniation were included. Bilateral biopsies of paravertebral muscles were obtained at the apical vertebral level for patients and at L5 level for controls. The concave and convex expression of PAX3 was compared between the two groups. The AIS patients were evaluated with magnetic resonance imaging scan of the spine at the apex. The muscle volume of apical paravertebral muscles were measured and compared between concave and convex side. Correlation between concave/convex PAX3 expression ratio and concave/convex muscle volume ratio was analyzed.
AIS patients were found to have significantly lower PAX3 expression than controls (p < 0.001). Moreover, PAX3 expression on the concave side was lower than that on the convex side in AIS (p = 0.003). The muscle volume on the concave side was smaller than convex side as well (p = 0.001). The PAX3 expression ratio was significantly correlated with the muscle volume ratio (r = 0.745, p = 0.013); however, there was no significant correlation between PAX3 expression ratio and Cobb angle (r = 0.284, p = 0.427).
The PAX3 muscle expression and paravertebral muscle volume were asymmetric in AIS patients, and the expression ratio was positively related with the muscle volume ratio. These findings suggested PAX3 might have functional role in the development of AIS via regulating development of paravertebral muscle. These slides can be retrieved under Electronic Supplementary Material.
本研究旨在探讨 PAX3 在青少年特发性脊柱侧凸（AIS）患者双侧椎旁肌中的表达，并进一步阐明其与椎旁肌体积和脊柱侧凸严重程度的关系。
纳入 10 例 AIS 患者和 10 例年龄匹配的腰椎间盘突出症患者。在 AIS 患者的顶椎水平，在对照组的 L5 水平获取双侧椎旁肌活检。比较两组患者的 PAX3 凹侧和凸侧的表达情况。对 AIS 患者进行脊柱磁共振成像扫描，评估脊柱顶椎。测量并比较顶椎双侧椎旁肌的肌肉体积。分析凹侧/凸侧 PAX3 表达比值与凹侧/凸侧肌肉体积比值的相关性。
与对照组相比，AIS 患者的 PAX3 表达明显降低（p<0.001）。此外，AIS 患者凹侧的 PAX3 表达也低于凸侧（p=0.003）。凹侧的肌肉体积也小于凸侧（p=0.001）。PAX3 表达比值与肌肉体积比值呈显著正相关（r=0.745，p=0.013）；然而，PAX3 表达比值与 Cobb 角无显著相关性（r=0.284，p=0.427）。
AIS 患者的 PAX3 肌肉表达和椎旁肌体积不对称，表达比值与肌肉体积比值呈正相关。这些发现提示 PAX3 可能通过调节椎旁肌的发育在 AIS 的发病机制中发挥作用。这些幻灯片可在电子补充材料中检索。
