哈氏 Hammondia 在培养的牛和羊细胞中的发育。

Development of Hammondia heydorni in cultured bovine and ovine cells.

作者信息

Speer C A, Dubey J P, Blixt J A, Blagburn B

机构信息

Veterinary Research Laboratory, Montana State University, Bozeman 59717.

出版信息

J Protozool. 1988 Aug;35(3):352-6. doi: 10.1111/j.1550-7408.1988.tb04105.x.

DOI:10.1111/j.1550-7408.1988.tb04105.x

PMID:3183994

Abstract

Sporozoites were excysted from oocysts of Hammondia heydorni obtained from a naturally-infected dog and inoculated into monolayer cultures of bovine pulmonary artery endothelial cells (CPA), Madin-Darby bovine kidney (MDBK) cells, bovine monocytes (M617), or ovine monocytes (WOMO). Sporozoites penetrated all four cell lines and underwent asexual reproduction by endodyogeny (as determined by electron microscopy) to form cyst-like structures at four to nine days after sporozoite inoculation (DAI). At 4-10 DAI, considerably more zoites were harvested from M617 cultures (80.1 x 10(6) zoites) than from CPA (17.4 x 10(6], MDBK (47.3 x 10(6], and WOMO (53.5 x 10(6]. Little or no parasite multiplication occurred at 10-16 DAI. Zoites harvested at 7 DAI and transferred to freshly prepared cultures did not penetrate cells nor develop further.

摘要

子孢子从一只自然感染犬的海氏哈蒙德球虫卵囊中逸出，并接种到牛肺动脉内皮细胞（CPA）、马-达二氏牛肾（MDBK）细胞、牛单核细胞（M617）或绵羊单核细胞（WOMO）的单层培养物中。子孢子侵入所有这四种细胞系，并通过内二分裂进行无性繁殖（通过电子显微镜确定），在接种子孢子后4至9天形成囊样结构。在接种后4至10天，从M617培养物中收获的子体（80.1×10⁶个子体）比从CPA（17.4×10⁶）、MDBK（47.3×10⁶）和WOMO（53.5×10⁶）培养物中收获的要多得多。在接种后10至16天，几乎没有或没有寄生虫增殖。在接种后7天收获的子体转移到新制备的培养物中，既不侵入细胞也不再进一步发育。