Tercan Vocational School, Erzincan Binali Yıldırım University, Erzincan, Turkey; Aquatic Biotechnology Laboratory, Fisheries Faculty, Atatürk University, Erzurum, Turkey.
Aquatic Biotechnology Laboratory, Fisheries Faculty, Atatürk University, Erzurum, Turkey; Aquaculture Department, Fisheries Faculty, Atatürk University, Erzurum, Turkey.
Neurotoxicology. 2020 Mar;77:51-59. doi: 10.1016/j.neuro.2019.12.010. Epub 2019 Dec 17.
Nano-sized plastic particles formed from both daily use plastics and its debris have become a potential health and environmental problem due to features such as transportation through food webs and maternal transfer. Although many studies on the toxicity of plastics exist more detailed and molecular studies are needed to evaluate and review the effects of plastics especially in nano-size range. For this purpose, we have microinjected polystyrene nanoplastics (PNP) (20 nm) to the zebrafish embryo, which is one of the best model organisms for developmental toxicity studies, to simulate intake with food or maternal. Survival, hatching and malformations evaluated during the experimental period (120 h). Moreover, we have aimed to put forth the presence of reactive oxygen species (ROS) and apoptosis signalling accumulation in the body in addition to bioaccumulation of PNP and immunochemical toxicity (8-OHdG) on the brain of zebrafish larvae at the 120th hour. According to results, it has been demonstrated that 20 nm diameter PNP can reach the brain and bioaccumulate there, moreover lead to oxidative DNA damage in the brain regions where it bioaccumulates. Here we have also imaged the PNP from a vertebrate brain via transmission electron microscopy (TEM) for the first time. As a result of these, it has been detected increasing mortality and prevailing abnormalities in addition to excessive ROS and apoptosis in especially the brain. As a conclusion, obtained data have suggested that precautions, on the use and contamination of the plastic product, to be taken during both pregnancy and baby care/feeding are important for the health of the baby in future.
由于能够通过食物链和母体转移等特性进行迁移，由日常使用的塑料及其碎片形成的纳米级塑料颗粒已经成为一个潜在的健康和环境问题。尽管已经有许多关于塑料毒性的研究，但需要更详细和分子水平的研究来评估和审查塑料的影响，尤其是在纳米尺寸范围内。为此，我们将聚苯乙烯纳米塑料（PNP）（20nm）微注射到斑马鱼胚胎中，斑马鱼胚胎是发育毒性研究的最佳模式生物之一，以模拟通过食物或母体摄入。在实验期间（120 小时）评估了存活率、孵化率和畸形率。此外，我们旨在提出在 120 小时时，体内 PNP 的生物累积、活性氧（ROS）和细胞凋亡信号的积累以及免疫化学毒性（8-OHdG）在斑马鱼幼虫大脑中的存在。结果表明，20nm 直径的 PNP 可以到达大脑并在那里累积，并且导致其累积的大脑区域中的 DNA 氧化损伤。我们还首次通过透射电子显微镜（TEM）对来自脊椎动物大脑的 PNP 进行了成像。由于这些原因，除了大脑中过量的 ROS 和细胞凋亡外，还检测到死亡率增加和普遍存在的异常。总之，获得的数据表明，在怀孕期间和婴儿护理/喂养期间，对塑料产品的使用和污染采取预防措施对于婴儿未来的健康非常重要。
