miR-140-5p 靶向 FGF9 并抑制喉鳞状细胞癌细胞生长。
miR-140-5p targeted FGF9 and inhibited the cell growth of laryngeal squamous cell carcinoma.
机构信息
Department of Otolaryngology, Liaocheng People's Hospital and EENT Hospital, Liaocheng Clinical School of Taishan Medical University, Liaocheng, 252000 Shandong, P.R. China.
Department of Otolaryngology Head and Neck, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou, 510080 Guangdong, P.R. China.
出版信息
Biochem Cell Biol. 2020 Apr;98(2):83-89. doi: 10.1139/bcb-2018-0351. Epub 2019 Dec 23.
Increasing evidence has suggested that microRNAs (miRNAs) play critical roles in the initiation and development of cancers. Here, we found that miR-140-5p was significantly downregulated in both laryngeal squamous cell carcinoma (LSCC) tissues and cell lines. Decreased expression of miR-140-5p was significantly associated with the metastasis of LSCC. Overexpression of miR-140-5p inhibited proliferation and induced apoptosis of LSCC cells. Mechanistically, the fibroblast growth factor 9 (FGF9) was identified as the target of miR-140-5p. miR-140-5p bound the 3'-untranslated region (3'-UTR) of FGF9 and suppressed the expression of FGF9 in LSCC cells. Additionally, the level of FGF9 was upregulated in LSCC tissues and negatively correlated with the expression of miR-140-5p. Restoration of FGF9 attenuated the suppressive role of miR-140-5p in regulating the growth of LSCC cells. Collectively, these results indicated that the tumor suppressive function of miR-140-5p in LSCC was partially exercised by modulating the expression of FGF9.
越来越多的证据表明 microRNAs（miRNAs）在癌症的发生和发展中起着关键作用。在这里，我们发现 miR-140-5p 在喉鳞状细胞癌（LSCC）组织和细胞系中均显著下调。miR-140-5p 的表达降低与 LSCC 的转移显著相关。miR-140-5p 的过表达抑制了 LSCC 细胞的增殖并诱导了细胞凋亡。机制上，成纤维细胞生长因子 9（FGF9）被鉴定为 miR-140-5p 的靶标。miR-140-5p 结合 FGF9 的 3'非翻译区（3'-UTR）并抑制 LSCC 细胞中 FGF9 的表达。此外，LSCC 组织中 FGF9 的水平上调，与 miR-140-5p 的表达呈负相关。FGF9 的恢复减弱了 miR-140-5p 对调节 LSCC 细胞生长的抑制作用。综上所述，这些结果表明 miR-140-5p 在 LSCC 中的肿瘤抑制功能部分通过调节 FGF9 的表达来发挥作用。
Zotero 插件