研究中东呼吸综合征冠状病毒的进化适应性。

Studying Evolutionary Adaptation of MERS-CoV.

作者信息

Letko Michael, Munster Vincent

机构信息

Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institutes of Health, Hamilton, MT, USA.

出版信息

Methods Mol Biol. 2020;2099:3-8. doi: 10.1007/978-1-0716-0211-9_1.

DOI:10.1007/978-1-0716-0211-9_1

PMID:31883083

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7121928/

Abstract

Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730-1737, 2018.

摘要

强制病毒适应是一种用于研究病毒如何克服各种降低病毒复制的选择压力的强大技术。在此，我们描述了中东呼吸综合征冠状病毒（MERS-CoV）体外连续传代的方法，以筛选出如Letko等人在《细胞报告》2018年第24卷第1730 - 1737页所述的能在半允许细胞系上增加复制的突变。