Hou Lele, Hou Jiafa, Zhou Zhenlei, Deng Yifeng, Yao Dawei
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210000, China.
Animal Husbandry and Veterinary Research Institute of Qingdao, Qingdao 266000, China.
Saudi J Biol Sci. 2020 Jan;27(1):288-295. doi: 10.1016/j.sjbs.2019.09.010. Epub 2019 Sep 13.
Thirty six 56-week-old ISA cage layers were divided into two groups randomly. The cage layers in control group (12 birds) and experiment group (24 birds) were respectively injected with 300 µL sodium chloride and 300 μg eucaryon recombinant plasmid pcDNA3.1(+)-chOPG. Eighty 56-week-old ISA cage layers were divided into group A, B, C and D randomly. Group A is for control group, while plasmid pcDNA3.1(+)-chOPG was injected to B, C, D groups in muscle at the dosage of 200 μg, 400 μg, 600 μg at 57, 59, 61, 63th weeks respectively. After the detection on the expression of chOPG protein after 3 h, it reached the peak at 7 d and then fell down. After 28 d, nothing was detected in the injected skeletal muscles. The other organs did not express exogenous chOPG protein. Plasmid in liver had the fastest metabolism. The pathological effects in main organs were not observed by histological section. The concentration of plasma calcium in B, C and D groups significantly decreased, while the activity of alkaline phosphatase was significantly improved, compared to control group. The total average value of abnormal and broken eggs of group C, D was significantly higher than those of group A. The bone biomechanical property and bone radiographic density of tibia and femur in experiment group were significantly higher than control group. Therefore, one conclusion is drawn that the expression of chOPG from foreign plasmid pcDNA3.1(+)-chOPG have contribute to bone formation, chOPG can increase bone density and strength by inhibiting bone resorption. Nevertheless, it was cleared out from cellular system in a short-term after intramuscular injection and cannot integrate into host chromosome genomic in cage layers. There were no pathological effects observed in the main tissues. It is believed that 200 μg plasmid pcDNA3.1(+)-chOPG should be within the safe range for application, because it can improve bone metabolism and will not affect the production of cage layer during the post cycle.
将36只56周龄的ISA笼养蛋鸡随机分为两组。对照组（12只鸡）和实验组（24只鸡）的笼养蛋鸡分别注射300μL氯化钠和300μg真核重组质粒pcDNA3.1(+)-chOPG。将80只56周龄的ISA笼养蛋鸡随机分为A、B、C、D四组。A组为对照组，而在第57、59、61、63周分别以200μg、400μg、600μg的剂量将质粒pcDNA3.1(+)-chOPG注射到B、C、D组的肌肉中。在3小时后检测chOPG蛋白的表达，其在7天时达到峰值，然后下降。28天后，在注射的骨骼肌中未检测到任何东西。其他器官未表达外源性chOPG蛋白。肝脏中的质粒代谢最快。通过组织学切片未观察到主要器官的病理影响。与对照组相比，B、C、D组血浆钙浓度显著降低，而碱性磷酸酶活性显著提高。C、D组异常蛋和破蛋的总平均值显著高于A组。实验组胫骨和股骨的骨生物力学性能和骨射线密度显著高于对照组。因此得出一个结论，即外源质粒pcDNA3.1(+)-chOPG的chOPG表达有助于骨形成，chOPG可通过抑制骨吸收增加骨密度和强度。然而，肌内注射后它在短期内从细胞系统中清除，并且不能整合到笼养蛋鸡的宿主染色体基因组中。在主要组织中未观察到病理影响。据信200μg质粒pcDNA3.1(+)-chOPG应在安全应用范围内，因为它可以改善骨代谢并且在产蛋后期不会影响笼养蛋鸡的生产。
Zotero 插件
