Center of Magnetic Resonance (CERM), University of Florence, via Luigi Sacconi, 6-50019 Sesto Fiorentino (FI), Italy.
Department of Experimental and Clinical Medicine, University of Florence, Largo Brambilla, 3-50134 Florence (FI), Italy.
Nutrients. 2019 Dec 27;12(1):86. doi: 10.3390/nu12010086.
Docosahexaenoic acid (DHA) has been reported to have a positive impact on many diet-related disease risks, including metabolic syndrome. Although many DHA-enriched foods have been marketed, the impact of different food matrices on the effect of DHA is unknown. As well, the possibility to enhance DHA effectiveness through the co-administration of other bioactives has seldom been considered. We evaluated DHA effects on the serum metabolome administered to volunteers at risk of metabolic syndrome as an ingredient of three different foods. Foods were enriched with DHA alone or in combination with oat beta-glucan or anthocyanins and were administered to volunteers for 4 weeks. Serum samples collected at the beginning and end of the trial were analysed by NMR-based metabolomics. Multivariate and univariate statistical analyses were used to characterize modifications in the serum metabolome and to evaluate bioactive-bioactive and bioactive-food matrix interactions. DHA administration induces metabolome perturbation that is influenced by the food matrix and the co-presence of other bioactives. In particular, when co-administered with oat beta-glucan, DHA induces a strong rearrangement in the lipoprotein profile of the subjects. The observed modifications are consistent with clinical results and indicate that metabolomics represents a possible strategy to choose the most appropriate food matrices for bioactive enrichment.
二十二碳六烯酸(DHA)已被报道对许多与饮食相关的疾病风险具有积极影响,包括代谢综合征。尽管已经推出了许多富含 DHA 的食品,但不同的食物基质对 DHA 效果的影响尚不清楚。此外,通过共同给予其他生物活性物质来增强 DHA 效果的可能性也很少被考虑。我们评估了 DHA 作为三种不同食物成分之一,对代谢综合征高危志愿者的血清代谢组的影响。食物单独或与燕麦β-葡聚糖或花青素一起富集 DHA,并在 4 周内给予志愿者。在试验开始和结束时收集的血清样本通过基于 NMR 的代谢组学进行分析。使用多变量和单变量统计分析来描述血清代谢组的修饰,并评估生物活性-生物活性和生物活性-食物基质相互作用。DHA 的给药会引起代谢组的扰动,而这种扰动受到食物基质和其他生物活性物质共同存在的影响。特别是,当与燕麦β-葡聚糖共同给药时,DHA 会强烈改变受试者的脂蛋白谱。观察到的修饰与临床结果一致,表明代谢组学代表了选择最适合生物活性富集的食物基质的一种可能策略。