从斑马鱼胚胎和成年鱼中分离总 RNA。
Isolation of Total RNA from Zebrafish Embryos and Adults.
出版信息
Cold Spring Harb Protoc. 2020 Jan 2;2020(1):101667. doi: 10.1101/pdb.prot101667.
This protocol, which uses a monophasic lysis reagent, is a rapid method for isolating high-quality total RNA from multiple samples. The protocol is a general procedure that can be used for isolating RNA from zebrafish embryos at any developmental stage. Embryos can be lysed directly in the monophasic lysis reagent without prior dechorionation. Typical yields from 25 6-hpf (hours postfertilization) embryos are 5-8 µg of RNA; 10-15 µg of RNA can be isolated from 25 24-hpf embryos.
本方案使用单相裂解试剂，是一种从多个样本中快速分离高质量总 RNA 的方法。该方案是一种通用程序，可用于分离任何发育阶段的斑马鱼胚胎中的 RNA。胚胎可以直接在单相裂解试剂中裂解，无需预先去卵膜。从 25 个 6 小时后受精（hours postfertilization，hpf）的胚胎中提取典型 RNA 的产量为 5-8 µg；从 25 个 24 小时后受精的胚胎中可以分离出 10-15 µg 的 RNA。
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