Hoffman D W, Jones-King K L, Ravaris C L, Edkins R D
Department of Psychiatry, Dartmouth Medical School, Hanover, New Hampshire 03756.
Anal Biochem. 1988 Aug 1;172(2):495-8. doi: 10.1016/0003-2697(88)90473-3.
Ketoconazole, cis-1-acetyl-4-[4[[2-(2,4-dichlorophenyl)-2-(1H-imidazol- 1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl]piperazine, a clinically used antifungal agent, is also an inhibitor of steroid hormone biosynthesis. A high-performance liquid chromatographic method is described which resolves ketoconazole with selectivity and high sensitivity provided by the use of electrochemical detection. Ketoconazole can be detected in high-performance liquid chromatography by electrochemical oxidation at a glassy carbon electrode at a potential of +1.0 V. Electrochemical detection offers improved sensitivity and selectivity over ultraviolet absorbance or fluorescence detection after derivatization. The method utilizes a volatile buffer system compatible with postcolumn analyses and an internal standard which is electrochemically active. This technique provides a simple method to assay ketoconazole. Ketoconazole can be detected in human plasma and saliva after a single oral therapeutic dose.
酮康唑，即顺-1-乙酰基-4-[4[[2-(2,4-二氯苯基)-2-(1H-咪唑-1-基甲基)-1,3-二氧戊环-4-基]甲氧基]苯基]哌嗪，是一种临床使用的抗真菌剂，也是类固醇激素生物合成的抑制剂。本文描述了一种高效液相色谱法，该方法通过使用电化学检测实现了酮康唑的选择性分离和高灵敏度检测。酮康唑在高效液相色谱中可通过在玻碳电极上于+1.0 V电位下进行电化学氧化来检测。与紫外吸收或衍生化后的荧光检测相比，电化学检测具有更高的灵敏度和选择性。该方法采用了与柱后分析兼容的挥发性缓冲系统以及具有电化学活性的内标。该技术为测定酮康唑提供了一种简单的方法。单次口服治疗剂量后，可在人血浆和唾液中检测到酮康唑。
