Department of Oral and Maxillofacial Pathology and Oral Microbiology, Post Graduate Institute of Dental Sciences, Pt. BD Sharma University of Health Sciences, Rohtak, Haryana, India.
Department of General Pathology, Post Graduate Institute of Medical Sciences, Pt. BD Sharma University of Health Sciences, Rohtak, Haryana, India.
Head Neck Pathol. 2020 Sep;14(3):749-757. doi: 10.1007/s12105-019-01114-1. Epub 2020 Jan 4.
The cancer stem cells deliver uncontrolled proliferative capacity within the tumor imparting to increasing size while epithelial mesenchymal transition adds to the invasive potential. Studies using specific markers elucidating the role of these phenomena may bring advancement in the targeted therapy of tumor. SOX2 and OCT4 are two among few stem cell markers indicative of proliferative potential and WNT5A is an epithelial mesenchymal transition marker indicative of invasive potential. We aimed to determine the association between expression of SOX2, OCT4 and WNT5A in oral epithelial dysplasia, oral squamous cell carcinoma and normal oral mucosa. 20 cases of oral squamous cell carcinoma, 20 cases of oral epithelial dysplasia (leukoplakia with dysplasia) and 25 normal oral mucosa tissues specimens were immunohistochemically stained to assess SOX2, OCT4 and WNT5A expression. SOX2 expression was higher in oral squamous cell carcinoma than in oral epithelial dysplasia and very low in normal oral mucosa. OCT4 was very low in oral squamous cell carcinoma and oral epithelial dysplasia when compared to SOX2, while negative in normal tissues. Co-expression of SOX2 and OCT4 showed statistically non-significant difference for tumor proliferation. WNT5A expression was found to be increasing from normal oral mucosa to oral epithelial dysplasia and oral squamous cell carcinoma. In conformity with present study, SOX2 itself can act as a potential marker for proliferation in tumor cells while OCT4 has non-significant role in regulation of tumor behavior in oral squamous cell carcinoma as well as in oral epithelial dysplasia. WNT5A can be a putative marker in studying invasive potential of oral squamous cell carcinoma.
肿瘤中的癌症干细胞赋予其不受控制的增殖能力，从而导致肿瘤不断增大，而上皮间质转化则增加了侵袭能力。使用特定标志物研究这些现象的作用可能会促进肿瘤靶向治疗的发展。SOX2 和 OCT4 是少数具有增殖潜能的干细胞标志物中的两种，而 WNT5A 是具有侵袭潜能的上皮间质转化标志物。我们旨在确定 SOX2、OCT4 和 WNT5A 在口腔上皮异型增生、口腔鳞状细胞癌和正常口腔黏膜中的表达之间的关系。免疫组织化学染色 20 例口腔鳞状细胞癌、20 例口腔上皮异型增生（伴异型增生的白斑）和 25 例正常口腔黏膜组织标本，评估 SOX2、OCT4 和 WNT5A 的表达。SOX2 在口腔鳞状细胞癌中的表达高于口腔上皮异型增生，在正常口腔黏膜中表达非常低。与 SOX2 相比，OCT4 在口腔鳞状细胞癌和口腔上皮异型增生病理中表达非常低，而在正常组织中则为阴性。SOX2 和 OCT4 的共表达对肿瘤增殖无统计学显著差异。从正常口腔黏膜到口腔上皮异型增生和口腔鳞状细胞癌，WNT5A 的表达逐渐增加。与本研究一致，SOX2 本身可以作为肿瘤细胞增殖的潜在标志物，而 OCT4 在口腔鳞状细胞癌和口腔上皮异型增生病理中对肿瘤行为的调节作用不显著。WNT5A 可能是研究口腔鳞状细胞癌侵袭潜能的一个有前途的标志物。
