不同光质处理对番茄叶片中 microRNAs 表达的影响。
Differential expression of microRNAs in tomato leaves treated with different light qualities.
机构信息
Vegetable and Flower Research Institute of Shandong Academy of Agricultural Sciences / Shandong Key Laboratory of Greenhouse Vegetable Biology / Shandong Branch of National Vegetable Improvement Center / Vegetable Science Observation and Experimental Station in Huang-Huai District of the Ministry of Agriculture, Jinan, 250100, China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai An, 271018, China.
出版信息
BMC Genomics. 2020 Jan 13;21(1):37. doi: 10.1186/s12864-019-6440-4.
BACKGROUND
Light is the main source of energy and, as such, is one of the most important environmental factors for plant growth, morphogenesis, and other physiological responses. MicroRNAs (miRNAs) are endogenous non-coding RNAs that contain 21-24 nucleotides (nt) and play important roles in plant growth and development as well as stress responses. However, the role of miRNAs in the light response is less studied. We used tomato seedlings that were cultured in red light then transferred to blue light for 2 min to identify miRNAs related to light response by high-throughput sequencing.
RESULTS
A total of 108 known miRNAs and 141 predicted novel miRNAs were identified in leaf samples from tomato leaves treated with the different light qualities. Among them, 15 known and 5 predicted novel miRNAs were differentially expressed after blue light treatment compared with the control (red light treatment). KEGG enrichment analysis showed that significantly enriched pathways included zeatin biosynthesis (ko00908), homologous recombination (ko03440), and plant hormone signal transduction (ko04075). Zeatin biosynthesis and plant hormone signal transduction are related to plant hormones, indicating that plant hormones play important roles in the light response.
CONCLUSION
Our results provide a theoretical basis for further understanding the role of miRNAs in the light response of plants.
背景
光作为主要的能量来源，是植物生长、形态发生和其他生理反应的最重要环境因素之一。MicroRNAs（miRNAs）是含有 21-24 个核苷酸（nt）的内源性非编码 RNA，在植物生长发育和应激反应中发挥重要作用。然而，miRNAs 在光响应中的作用研究较少。我们使用在红光下培养然后转移到蓝光下 2 分钟的番茄幼苗，通过高通量测序来鉴定与光反应相关的 miRNAs。
结果
在不同光质处理的番茄叶片样本中，共鉴定出 108 个已知的 miRNAs 和 141 个预测的新 miRNAs。其中，与对照（红光处理）相比，15 个已知和 5 个预测的新 miRNAs 在蓝光处理后差异表达。KEGG 富集分析表明，显著富集的途径包括玉米素生物合成（ko00908）、同源重组（ko03440）和植物激素信号转导（ko04075）。玉米素生物合成和植物激素信号转导与植物激素有关，表明植物激素在光响应中起重要作用。
结论
我们的研究结果为进一步了解 miRNAs 在植物光响应中的作用提供了理论基础。
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