[二苯乙烯苷抑制紫外线B诱导的人皮肤成纤维细胞光老化]
[Stilbene glucoside inhibits ultraviolet radiation B-induced photoaging in human skin fibroblasts].
作者信息
Xu Meijiao, Wang Yifeng
机构信息
Department of Dermatology, Suzhou Dushuhu Public Hospital, Dushuhu Public Hospital Affiliated to Soochow University, Suzhou 215000, China.
The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2019 Dec 25;48(6):625-630. doi: 10.3785/j.issn.1008-9292.2019.12.06.
OBJECTIVE
To study the effects of tetrahydroxy stilbene-2-O-β-D-glucoside (TSG) on stress-induced premature senescence of human skin fibroblasts (HSF) exposed to ultraviolet radiation B (UVB) and its possible mechanism.
METHODS
HSFs were repeatedly exposed to UVB at a subcytotoxic level. TSG treatment (0.02, 0.10 and 0.50 mmol/L) was given immediately after each irradiation. The HSFs were divided into six groups:blank control group, model group, UVB+0.02 mmol/L TSG group, UVB+0.10 mmol/L TSG group, UVB+0.50 mmol/L TSG and TSG group (0.50 mmol/L). Cell counting kit-8 (CCK-8) was used to evaluate the proliferative activity of cells; senescence-associated-β-galactosidase (SA-β-gal) staining was performed to estimate the degree of premature senescence in cells; TBA method and WST-1 method were used to detect intracellular malondialdehyde (MDA) and superoxide dismutase (SOD) activities; and ELISA was applied to quantify the secretion level of matrix metalloproteinase1 (MMP-1) in cultured supernatant.
RESULTS
Compared with the blank control group, the proliferative activity and SOD level in the model group decreased (<0.05), while the percentage of SA-β-gal-positive cells, MDA and MMP-1 levels increased (<0.05 or <0.01). Compared with the model group, the proliferative activity and SOD level increased in UVB+TSG groups (all <0.05), and the percentage of SA-β-gal-positive cells, MDA and MMP-1 levels decreased (<0.05 or <0.01).
CONCLUSIONS
TSG can inhibit UVB-induced premature senescence of HSF, which may be related to the inhibition of oxidative stress and high expression of MMP-1.
目的
研究四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG）对紫外线B（UVB）照射下人皮肤成纤维细胞（HSF）应激诱导的早衰的影响及其可能机制。
方法
将HSF以亚细胞毒性水平反复暴露于UVB。每次照射后立即给予TSG处理（0.02、0.10和0.50 mmol/L）。将HSF分为六组：空白对照组、模型组、UVB + 0.02 mmol/L TSG组、UVB + 0.10 mmol/L TSG组、UVB + 0.50 mmol/L TSG组和TSG组（0.50 mmol/L）。采用细胞计数试剂盒-8（CCK-8）评估细胞增殖活性；进行衰老相关β-半乳糖苷酶（SA-β-gal）染色以评估细胞早衰程度；采用硫代巴比妥酸（TBA）法和WST-1法检测细胞内丙二醛（MDA）和超氧化物歧化酶（SOD）活性；采用酶联免疫吸附测定（ELISA）法测定培养上清液中基质金属蛋白酶1（MMP-1）的分泌水平。
结果
与空白对照组相比，模型组细胞增殖活性和SOD水平降低（<0.05），而SA-β-gal阳性细胞百分比、MDA和MMP-1水平升高（<0.05或<0.01）。与模型组相比，UVB + TSG组细胞增殖活性和SOD水平升高（均<0.05），SA-β-gal阳性细胞百分比、MDA和MMP-1水平降低（<0.05或<0.01）。
结论
TSG可抑制UVB诱导的HSF早衰，这可能与抑制氧化应激和MMP-1的高表达有关。
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