与人脂肪来源干细胞共培养后提高人胰岛质量。
Improvement of human pancreatic islet quality after co-culture with human adipose-derived stem cells.
机构信息
Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil; Universidade Federal do Rio Grande do Sul, Faculty of Medicine, Department of Internal Medicine, Graduate Program in Medical Sciences: Endocrinology, Porto Alegre, RS, Brazil.
Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clınicas de Porto Alegre, Porto Alegre, Rio Grande do Sul (RS), Brazil.
出版信息
Mol Cell Endocrinol. 2020 Apr 5;505:110729. doi: 10.1016/j.mce.2020.110729. Epub 2020 Jan 21.
The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h: 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality.
本研究旨在探讨人胰岛与脂肪干细胞（ASCs）共培养是否能改善胰岛质量，并评估哪些因素在 ASCs 对抗胰岛功能障碍的保护作用中发挥作用。将胰岛和 ASCs 在三个实验组中培养 24、48 和 72 小时：1）胰岛与 ASC 单层的间接共培养（胰岛/ASCs）；2）胰岛单独培养；3）ASCs 单独培养。与 ASCs 共培养可提高胰岛在所有分析的培养时间点的活力和功能。与胰岛单独培养相比，胰岛/ASC 组的上清液中 VEGFA、HGF、IL6、IL8、IL10、CCL2、IL1B 和 TNF 蛋白水平升高，主要在 24 小时后升高。此外，与胰岛单独培养相比，共培养条件下胰岛中 VEGFA、IL6、CCL2、HIF1A、XIAP、CHOP 和 NFKBIA 基因的表达存在差异。总之，胰岛与 ASCs 的共培养促进了胰岛质量的改善。
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