通过季铵盐和苯硼酸基团的表面修饰制备和应用 PGMA-DVB 微球。
Preparation and application of PGMA-DVB microspheres via surface-modification with quaternary and phenylboronic acid moiety.
机构信息
Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China.
Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China; State Key Laboratory of Bio-Fibers and Eco-Textiles, Qingdao University, Qingdao 266071, China.
出版信息
Colloids Surf B Biointerfaces. 2020 Apr;188:110807. doi: 10.1016/j.colsurfb.2020.110807. Epub 2020 Jan 17.
Porous cross-linked poly (glycidyl methacrylate-divinylbenzene) (PGMA-DVB) particles (7.42 ± 0.24 μm in diameter) were prepared by an improved two-step seed swelling polymerization method. The PGMA-DVB particles were further modified with poly (allylamine hydrochloride) (PAH) and 4-(bromomethyl) phenylboronic acid (BPA), which were used as high performance liquid chromatography (HPLC) filler for nucleotides separation. The liquid chromatographic column packing materials successfully achieved complete separation of nucleotides mixture or deoxynucleotides mixture based on non-polar adsorption, hydrogen bonding interaction and electrostatic adsorption. Reckoning on the chemical structure of BPA, nucleotides and deoxynucleotides with same base group were also separated. The column packing materials were durable after over 100 time running or 7 days. It presents a kind of new notion for the separation of nucleotides by HPLC.
多孔交联聚（甲基丙烯酸缩水甘油酯-二乙烯基苯）（PGMA-DVB）颗粒（直径为 7.42±0.24μm）采用改进的两步种子溶胀聚合方法制备。PGMA-DVB 颗粒进一步用聚（盐酸烯丙胺）（PAH）和 4-（溴甲基）苯硼酸（BPA）改性，用作核苷酸分离的高效液相色谱（HPLC）填充剂。基于非极性吸附、氢键相互作用和静电吸附，液相色谱柱填充材料成功实现了核苷酸混合物或脱氧核苷酸混合物的完全分离。根据 BPA 的化学结构，碱基相同的核苷酸和脱氧核苷酸也被分离。该柱填充材料在 100 多次运行或 7 天后仍具有耐用性。它为 HPLC 分离核苷酸提供了一种新的概念。
Zotero 插件