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提高在人体生理溶液中运行的介体型葡萄糖酶电极的操作稳定性。

Improved operational stability of mediated glucose enzyme electrodes for operation in human physiological solutions.

机构信息

School of Chemistry & Ryan Institute, National University of Ireland Galway, University Road, Galway, Ireland.

School of Chemistry & Ryan Institute, National University of Ireland Galway, University Road, Galway, Ireland.

出版信息

Bioelectrochemistry. 2020 Jun;133:107460. doi: 10.1016/j.bioelechem.2020.107460. Epub 2020 Jan 21.

Abstract

Stability of glucose-oxidising enzyme electrodes is affected by substances in physiological solutions, hampering deployment as long-term implantable biosensors or fuel cells. The performance of Nafion over-coated enzyme electrodes, consisting of multiwalled carbon nanotubes and flavin adenine dinucleotide-dependent glucose dehydrogenase (FADGDH) or glucose oxidase (GOx) crosslinked with osmium-complex based redox polymer, was compared to uncoated electrodes in presence of uric acid and artificial plasma. Nafion over-coating resulted in lower glucose oxidation current densities compared to no over-coating. The highest initial current density for Nafion over-coated electrodes in artificial plasma in 100 mM glucose was 8.0 ± 2.0 mA cm for GOx electrodes with 0.5% w/v Nafion coating. These electrodes retained 83% of initial current after 12 h continuous operation in artificial plasma while similarly prepared FADGDH electrodes retained 58% signal. This is compared to retention of only 73% or 31% observed for GOx or FADGDH electrodes in artificial plasma with no Nafion membrane. Enzyme electrodes over-coated with Nafion maintain improved signal stability when tested continuously in the presence of uric acid, identified as being the main contributing substance to FADGDH enzyme electrode instability, showing promise for application to continuous use glucose-oxidising enzyme electrodes.

摘要

葡萄糖氧化酶电极的稳定性会受到生理溶液中物质的影响,这阻碍了它们作为长期植入式生物传感器或燃料电池的应用。本文比较了多壁碳纳米管和黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶(FADGDH)或葡萄糖氧化酶(GOx)交联的基于锇复合物的氧化还原聚合物修饰的酶电极与未修饰电极在尿酸和人工血浆中的性能。与未修饰相比,Nafion 修饰会导致葡萄糖氧化电流密度降低。在 100mM 葡萄糖的人工血浆中,GOx 电极初始电流密度最高,为 8.0±2.0mA cm,Nafion 涂层质量分数为 0.5%。这些电极在人工血浆中连续运行 12 小时后保留了初始电流的 83%,而类似制备的 FADGDH 电极保留了 58%的信号。相比之下,在没有 Nafion 膜的人工血浆中,GOx 或 FADGDH 电极的保留率仅为 73%或 31%。在尿酸存在的情况下连续测试时,用 Nafion 修饰的酶电极保持了更好的信号稳定性,尿酸被认为是导致 FADGDH 酶电极不稳定性的主要物质,这为连续使用葡萄糖氧化酶电极的应用提供了希望。

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