Klang Johanna, Szewzyk Ulrich, Bock Daniel, Theuerl Susanne
Leibniz Institute for Agricultural Engineering and Bioeconomy, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
Department of Environmental Microbiology, Technische Universität Berlin, Ernst-Reuter-Platz 1, 10587 Berlin, Germany.
Microorganisms. 2020 Jan 25;8(2):169. doi: 10.3390/microorganisms8020169.
In this study the response of biogas-producing microbiomes to a profound feedstock change was investigated. The microbiomes were adapted to the digestion of either 100% sugar beet, maize silage, or of the silages with elevated amounts of total ammonium nitrogen (TAN) by adding ammonium carbonate or animal manure. The feedstock exchange resulted in a short-range decrease or increase in the biogas yields according to the level of chemical feedstock complexity. Fifteen taxa were found in all reactors and can be considered as generalists. Thirteen taxa were detected in the reactors operated with low TAN and six in the reactors with high TAN concentration. Taxa assigned to the phylum and to the order increased with the exchange to sugar beet silage, indicating an affinity to easily degradable compounds. The recorded TAN-sensitive taxa (phylum ) showed no specific affinity to maize or sugar beet silage. The archaeal community remained unchanged. The reported findings showed a smooth adaptation of the microbial communities, without a profound negative impact on the overall biogas production indicating that the two feedstocks, sugar beet and maize silage, potentially do not contain chemical compounds that are difficult to handle during anaerobic digestion.
在本研究中,对产沼气微生物群落对原料的深度变化的响应进行了调查。微生物群落通过添加碳酸铵或动物粪便,适应了100%甜菜、玉米青贮饲料或总铵氮(TAN)含量升高的青贮饲料的消化。根据化学原料复杂性水平,原料交换导致沼气产量短期下降或增加。在所有反应器中发现了15个分类单元,可视为通才。在低TAN运行的反应器中检测到13个分类单元,在高TAN浓度的反应器中检测到6个分类单元。归属于门 和纲 的分类单元随着向甜菜青贮饲料的交换而增加,表明对易降解化合物具有亲和力。记录的对TAN敏感的分类单元(门 )对玉米或甜菜青贮饲料没有特定亲和力。古菌群落保持不变。报告的研究结果表明微生物群落能顺利适应,对整体沼气生产没有深远的负面影响,这表明甜菜和玉米青贮这两种原料在厌氧消化过程中可能不含有难以处理的化合物。