School of life sciences, Anhui Agricultural University, Hefei 230036, People's Republic of China.
Nanotechnology. 2020 May 1;31(18):185101. doi: 10.1088/1361-6528/ab70fb. Epub 2020 Jan 28.
Diseases caused by pathogenic bacilli pose an increasing threat to human health. A common feature of these bacteria is a complete cell wall; therefore, drugs that can penetrate this protective barrier could be used as a novel approach for treating these infections. Here we present a simple method for synthesizing a silica mesoporous material loaded with cadmium selenide (CdSe) and chlorogenic acid. Using UV-visible, fluorescence, and infrared imaging in combination with transmission electron microscopy, it was shown that CdSe and chlorogenic acid could be successfully embedded in the mesopores of silica nanoparticles (CSC NPs), and these NPs presented with a strong fluorescence, uniform size, and good dispersion. Additionally, the results of these analyses indicated that the fluorescence of the CSC NPs was localized within the cells of Escherichia coli and Bacillus subtilis, signifying that these NPs could breach the cell wall and enter the cells of these two bacilli. Additional assessments found that these CSC NPs inhibited the proliferation of the bacteria by disrupting the cell wall, and this was most likely due to the overproduction of reactive oxygen species induced by chlorogenic acid. Importantly, histopathology analysis indicated that the CSC NPs had limited side effects and high biocompatibility.
致病杆菌引起的疾病对人类健康构成越来越大的威胁。这些细菌的一个共同特征是完整的细胞壁；因此，能够穿透这种保护屏障的药物可以被用作治疗这些感染的新方法。在这里，我们提出了一种简单的方法来合成负载硒化镉（CdSe）和绿原酸的硅质介孔材料。通过紫外-可见、荧光和红外成像结合透射电子显微镜，表明 CdSe 和绿原酸可以成功地嵌入硅纳米颗粒（CSC NPs）的介孔中，并且这些 NPs 呈现出强荧光、均匀的尺寸和良好的分散性。此外，这些分析的结果表明，CSC NPs 的荧光定位于大肠杆菌和枯草芽孢杆菌的细胞内，表明这些 NPs 可以穿透细胞壁并进入这两种杆菌的细胞内。进一步的评估发现，这些 CSC NPs 通过破坏细胞壁抑制了细菌的增殖，这很可能是由于绿原酸诱导的活性氧过度产生。重要的是，组织病理学分析表明，CSC NPs 具有有限的副作用和高生物相容性。
