Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of Korea.
Physiol Meas. 2020 Mar 6;41(2):025009. doi: 10.1088/1361-6579/ab71f3.
The individual effects of plasma and red blood cells (RBCs) on the biophysical properties of blood can be monitored by measuring the erythrocyte sedimentation rate (ESR) and RBC deformability simultaneously. However, the previous methods require bulky and expensive facilities (i.e. microscope, high-speed camera, and syringe pump) to deliver blood or capture blood flows.
To resolve these issues, a simple method for sequential measurement of the ESR and RBC deformability is demonstrated by quantifying the cell-free volume (V ), cell-rich volume (V ), and blood volume (V ) inside an air-compressed syringe (ACS). A microfluidic device consists of multiple micropillar channels, an inlet, and outlet. After the ACS is filled with air (V = 0.4 ml) and a blood sample (V = 0.6 ml, hematocrit = 30%) sequentially, the ACS is fitted into the inlet. The cavity inside the ACS is compressed to V = 0.4 ml after closing the outlet with a stopper. A smartphone camera is employed to capture variations in the V , V , and V inside the ACS. The ESR index suggested in this study (ESR ) is obtained by dividing the V (t = t ) with an elapse of t . By removing the stopper, ΔV (ΔV = V [t = t ] - V ) is obtained and fitted as a two-term exponential model ([Formula: see text]. As a performance demonstration, the proposed method is employed to detect an ESR-enhanced blood sample, homogeneous hardened blood sample, and heterogeneous blood sample.
From the experimental results, it is found that the proposed method has the ability to detect various bloods by quantifying the ESR and two coefficients (a, b) simultaneously.
In conclusion, the present method can be effectively used to measure the ESR and RBC deformability in resource-limited settings.
通过同时测量红细胞沉降率（ESR）和红细胞变形性，可以监测血浆和红细胞（RBC）对血液生物物理特性的个体影响。然而，以前的方法需要庞大且昂贵的设备（即显微镜、高速摄像机和注射器泵）来输送血液或捕捉血液流动。
为了解决这些问题，通过量化无细胞体积（V ）、富含细胞的体积（V ）和空气压缩注射器（ACS）内的血液体积（V ），展示了一种用于顺序测量 ESR 和 RBC 变形性的简单方法。微流控装置由多个微柱通道、入口和出口组成。在 ACS 充满空气（V = 0.4 ml）和血液样本（V = 0.6 ml，血细胞比容 = 30%）之后，ACS 被装入入口。关闭出口上的塞子后，将 ACS 内部的腔室压缩至 V = 0.4 ml。智能手机摄像头用于捕获 ACS 内 V 、V 和 V 的变化。本研究中提出的 ESR 指数（ESR ）是通过将 V （t = t ）除以 t 的流逝时间来获得的。通过移除塞子，获得 ΔV （ΔV = V [t = t ] - V ）并拟合为双项指数模型([Formula: see text]。作为性能演示，该方法用于检测 ESR 增强的血液样本、均匀硬化的血液样本和异质血液样本。
从实验结果中发现，该方法通过同时量化 ESR 和两个系数（a，b），具有检测各种血液的能力。
总之，本方法可在资源有限的环境中有效测量 ESR 和 RBC 变形性。
